Recovery of live virus after storage at ambient temperature using ViveST™.

Published

Journal Article

BACKGROUND: A major impediment to performing virological field studies in developing nations is the lack of ultra-low freezers as well as the expense and difficulty of shipping frozen samples. A commercially available product, ViveST™, was developed to preserve nucleic acids at ambient temperature for use in specimen storage and transportation. However, its applications as a viral storage, transport and recovery device have not been evaluated. OBJECTIVE: To examine the ability of ViveST to preserve live virus following storage at ambient temperature. STUDY DESIGN: A panel of six viruses was stored at ambient temperature (~22°C) in ViveST with fetal bovine serum (FBS), or ViveST with minimal essential media (MEM) and compared with virus stored in universal transport media (M4RT), MEM, and FBS alone. Stored viruses included: human adenovirus (14p), dengue virus 2 (16608), echovirus 3 (Morrisey), human rhinovirus 15 (1734), Coxsackie virus B5 (Faulkner), and herpes simplex virus 1 (HF). After 7 days storage at ambient temperature, virus recovery was measured via titration using viral plaque assays or focus-forming unit assays. RESULTS: Viral titer studies indicate that ViveST with either FBS or M4RT preserved/recovered 5 different viruses for 1 week at ambient temperature. MEM preserved 4 viruses while FBS and ViveST with MEM preserved 3 viruses each. Statistical analyses indicate that M4RT and ViveST with FBS preserved significantly more virus than the other treatments. CONCLUSIONS: These data suggest that ViveST with either FBS or M4RT may be useful in field specimen collection scenarios where ultra-cold storage is not available.

Full Text

Duke Authors

Cited Authors

  • Barr, KL; Messenger, AM; Anderson, BD; Friary, JA; Heil, GL; Reece, K; Gray, GC

Published Date

  • January 2013

Published In

Volume / Issue

  • 56 / 1

Start / End Page

  • 57 - 61

PubMed ID

  • 23046621

Pubmed Central ID

  • 23046621

Electronic International Standard Serial Number (EISSN)

  • 1873-5967

Digital Object Identifier (DOI)

  • 10.1016/j.jcv.2012.09.005

Language

  • eng

Conference Location

  • Netherlands