A modified rapid method of nucleic acid isolation from suspension of matured virus: applied in restriction analysis of DNA from an adenovirus prototype strain and a patient isolate.

Published

Journal Article

This report describes a method for the isolation of nucleic acid from a suspension of matured virus. Nucleic acid (DNA) was isolated from a prototype strain of adenovirus type 7 and a clinical isolate of adenovirus type 7. Instead of the usual method of ultracentifugation, a filtration method was applied to concentrate the virus rapidly and nucleic acid was then isolated by a standard phenol/chloroform/isoamyl-alcohol extraction procedure. The DNA was found to be sufficiently purified to generate a reproducible restriction endonuclease digestion pattern. The clinical isolate of adenovirus type 7 revealed loss of restriction site for the endonuclease HindIII when compared with the prototype strain.

Full Text

Duke Authors

Cited Authors

  • Le, CT; Gray, GC; Poddar, SK

Published Date

  • June 2001

Published In

Volume / Issue

  • 50 / 6

Start / End Page

  • 571 - 574

PubMed ID

  • 11393295

Pubmed Central ID

  • 11393295

International Standard Serial Number (ISSN)

  • 0022-2615

Digital Object Identifier (DOI)

  • 10.1099/0022-1317-50-6-571

Language

  • eng

Conference Location

  • England