PCR based determination of mitochondrial DNA copy number in multiple species.
Publication
, Journal Article
Rooney, JP; Ryde, IT; Sanders, LH; Howlett, EH; Colton, MD; Germ, KE; Mayer, GD; Greenamyre, JT; Meyer, JN
Published in: Methods Mol Biol
2015
Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA (nucDNA) and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material and availability of specific PCR reagents.
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Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2015
Volume
1241
Start / End Page
23 / 38
Location
United States
Related Subject Headings
- Rats
- Polymerase Chain Reaction
- Mice
- Humans
- Gene Dosage
- Developmental Biology
- DNA, Mitochondrial
- Cell Nucleus
- Calibration
- Caenorhabditis elegans
Citation
APA
Chicago
ICMJE
MLA
NLM
Rooney, J. P., Ryde, I. T., Sanders, L. H., Howlett, E. H., Colton, M. D., Germ, K. E., … Meyer, J. N. (2015). PCR based determination of mitochondrial DNA copy number in multiple species. Methods Mol Biol, 1241, 23–38. https://doi.org/10.1007/978-1-4939-1875-1_3
Rooney, John P., Ian T. Ryde, Laurie H. Sanders, Evan H. Howlett, Meryl D. Colton, Kaylyn E. Germ, Greg D. Mayer, J Timothy Greenamyre, and Joel N. Meyer. “PCR based determination of mitochondrial DNA copy number in multiple species.” Methods Mol Biol 1241 (2015): 23–38. https://doi.org/10.1007/978-1-4939-1875-1_3.
Rooney JP, Ryde IT, Sanders LH, Howlett EH, Colton MD, Germ KE, et al. PCR based determination of mitochondrial DNA copy number in multiple species. Methods Mol Biol. 2015;1241:23–38.
Rooney, John P., et al. “PCR based determination of mitochondrial DNA copy number in multiple species.” Methods Mol Biol, vol. 1241, 2015, pp. 23–38. Pubmed, doi:10.1007/978-1-4939-1875-1_3.
Rooney JP, Ryde IT, Sanders LH, Howlett EH, Colton MD, Germ KE, Mayer GD, Greenamyre JT, Meyer JN. PCR based determination of mitochondrial DNA copy number in multiple species. Methods Mol Biol. 2015;1241:23–38.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2015
Volume
1241
Start / End Page
23 / 38
Location
United States
Related Subject Headings
- Rats
- Polymerase Chain Reaction
- Mice
- Humans
- Gene Dosage
- Developmental Biology
- DNA, Mitochondrial
- Cell Nucleus
- Calibration
- Caenorhabditis elegans