Skip to main content
Journal cover image

Controlled exosome release from the retinal pigment epithelium in situ.

Publication ,  Journal Article
Locke, CJ; Congrove, NR; Dismuke, WM; Bowen, TJ; Stamer, WD; McKay, BS
Published in: Exp Eye Res
December 2014

Retinal Pigment Epithelial cells (RPE) express both GPR143 and myocilin, which interact in a signal transduction-dependent manner. In heterologous systems, activation of GPR143 with ligand causes transient recruitment of myocilin to internalized receptors, which appears to be the entry point of myocilin to the endocytic pathway. In some but not all cells, myocilin also traffics through the multivesicular body (MVB) and is released on the surface of exosomes in a signal transduction-dependent fashion. Little is known regarding the role of exosomes in RPE, but they likely serve as a mode of communication between the RPE and the outer retina. In this study, we used posterior poles with retina removed from fresh human donor eyes as a model to test the relationship between GPR143, myocilin, and exosomes in an endogenous system. We isolated exosomes released by RPE using differential centrifugation of media conditioned by the RPE for 25 min, and then characterized the exosomes using nanoparticle tracking to determine the number and size of the exosomes. Next, we tested whether ligand stimulation of GPR143 using l-DOPA altered RPE exosome release. Finally, we investigated whether myocilin was present on the exosomes released by RPE and whether l-DOPA stimulation of GPR143 caused recruitment of myocilin to the endocytic pathway, as we have previously observed using cultured cells. Activation of GPR143 halted RPE exosome release, while simultaneously recruiting myocilin to the endocytic compartment. Together, our results indicate that GPR143 and myocilin function in a signal transduction system that can control exosome release from RPE.

Duke Scholars

Published In

Exp Eye Res

DOI

EISSN

1096-0007

Publication Date

December 2014

Volume

129

Start / End Page

1 / 4

Location

England

Related Subject Headings

  • Signal Transduction
  • Retinal Pigment Epithelium
  • Ophthalmology & Optometry
  • Humans
  • Exosomes
  • Cells, Cultured
  • 3212 Ophthalmology and optometry
  • 1113 Opthalmology and Optometry
  • 1109 Neurosciences
  • 1101 Medical Biochemistry and Metabolomics
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Locke, C. J., Congrove, N. R., Dismuke, W. M., Bowen, T. J., Stamer, W. D., & McKay, B. S. (2014). Controlled exosome release from the retinal pigment epithelium in situ. Exp Eye Res, 129, 1–4. https://doi.org/10.1016/j.exer.2014.10.010
Locke, Christina J., Nicole R. Congrove, W Michael Dismuke, Trent J. Bowen, W Daniel Stamer, and Brian S. McKay. “Controlled exosome release from the retinal pigment epithelium in situ.Exp Eye Res 129 (December 2014): 1–4. https://doi.org/10.1016/j.exer.2014.10.010.
Locke CJ, Congrove NR, Dismuke WM, Bowen TJ, Stamer WD, McKay BS. Controlled exosome release from the retinal pigment epithelium in situ. Exp Eye Res. 2014 Dec;129:1–4.
Locke, Christina J., et al. “Controlled exosome release from the retinal pigment epithelium in situ.Exp Eye Res, vol. 129, Dec. 2014, pp. 1–4. Pubmed, doi:10.1016/j.exer.2014.10.010.
Locke CJ, Congrove NR, Dismuke WM, Bowen TJ, Stamer WD, McKay BS. Controlled exosome release from the retinal pigment epithelium in situ. Exp Eye Res. 2014 Dec;129:1–4.
Journal cover image

Published In

Exp Eye Res

DOI

EISSN

1096-0007

Publication Date

December 2014

Volume

129

Start / End Page

1 / 4

Location

England

Related Subject Headings

  • Signal Transduction
  • Retinal Pigment Epithelium
  • Ophthalmology & Optometry
  • Humans
  • Exosomes
  • Cells, Cultured
  • 3212 Ophthalmology and optometry
  • 1113 Opthalmology and Optometry
  • 1109 Neurosciences
  • 1101 Medical Biochemistry and Metabolomics