Distinct and redundant roles of exonucleases in Cryptococcus neoformans: implications for virulence and mating.

Published

Journal Article

Opportunistic pathogens like Cryptococcus neoformans are constantly exposed to changing environments, in their natural habitat as well as when encountering a human host. This requires a coordinated program to regulate gene expression that can act at the levels of mRNA synthesis and also mRNA degradation. Here, we find that deletion of the gene encoding the major cytoplasmic 5'→3' exonuclease Xrn1p in C. neoformans has important consequences for virulence associated phenotypes such as growth at 37 °C, capsule and melanin. In an invertebrate model of cryptococcosis the alteration of these virulence properties corresponds to avirulence of the xrn1Δ mutant strains. Additionally, deletion of XRN1 impairs uni- and bisexual mating. On a molecular level, the absence of XRN1 is associated with the upregulation of other major exonuclease encoding genes (i.e. XRN2 and RRP44). Using inducible alleles of RRP44 and XRN2, we show that artificial overexpression of these genes alters LAC1 gene expression and mating. Our data thus suggest the existence of a complex interdependent regulation of exonuclease encoding genes that impact upon virulence and mating in C. neoformans.

Full Text

Duke Authors

Cited Authors

  • Wollschlaeger, C; Trevijano-Contador, N; Wang, X; Legrand, M; Zaragoza, O; Heitman, J; Janbon, G

Published Date

  • December 2014

Published In

Volume / Issue

  • 73 /

Start / End Page

  • 20 - 28

PubMed ID

  • 25267175

Pubmed Central ID

  • 25267175

Electronic International Standard Serial Number (EISSN)

  • 1096-0937

Digital Object Identifier (DOI)

  • 10.1016/j.fgb.2014.09.007

Language

  • eng

Conference Location

  • United States