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Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs

Publication ,  Journal Article
Nguyen, H; Badie, N; McSpadden, L; Pedrotty, D; Bursac, N
Published in: Methods in Molecular Biology
2014

Micropatterning is a powerful technique to control cell shape and position on a culture substrate. In this chapter, we describe the method to reproducibly create large numbers of micropatterned heterotypic cell pairs with defined size, shape, and length of cell-cell contact. These cell pairs can be utilized in patch clamp recordings to quantify electrical interactions between cardiomyocytes and non-cardiomyocytes. © 2014 Springer Science+Business Media New York.

Duke Scholars

Published In

Methods in Molecular Biology

DOI

ISSN

1064-3745

Publication Date

2014

Volume

1181

Start / End Page

249 / 262

Publisher

Humana Press Inc.

Related Subject Headings

  • Developmental Biology
  • 0601 Biochemistry and Cell Biology
  • 0399 Other Chemical Sciences
 

Citation

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ICMJE
MLA
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Nguyen, H., Badie, N., McSpadden, L., Pedrotty, D., & Bursac, N. (2014). Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs. Methods in Molecular Biology, 1181, 249–262. https://doi.org/10.1007/978-1-4939-1047-2-21
Nguyen, H., N. Badie, L. McSpadden, D. Pedrotty, and N. Bursac. “Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs.” Methods in Molecular Biology 1181 (2014): 249–62. https://doi.org/10.1007/978-1-4939-1047-2-21.
Nguyen H, Badie N, McSpadden L, Pedrotty D, Bursac N. Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs. Methods in Molecular Biology. 2014;1181:249–62.
Nguyen, H., et al. “Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs.” Methods in Molecular Biology, vol. 1181, Humana Press Inc., 2014, pp. 249–62. Scopus, doi:10.1007/978-1-4939-1047-2-21.
Nguyen H, Badie N, McSpadden L, Pedrotty D, Bursac N. Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs. Methods in Molecular Biology. Humana Press Inc.; 2014;1181:249–262.

Published In

Methods in Molecular Biology

DOI

ISSN

1064-3745

Publication Date

2014

Volume

1181

Start / End Page

249 / 262

Publisher

Humana Press Inc.

Related Subject Headings

  • Developmental Biology
  • 0601 Biochemistry and Cell Biology
  • 0399 Other Chemical Sciences