Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution.
Published
Journal Article
Although fluorescence microscopy provides a crucial window into the physiology of living specimens, many biological processes are too fragile, are too small, or occur too rapidly to see clearly with existing tools. We crafted ultrathin light sheets from two-dimensional optical lattices that allowed us to image three-dimensional (3D) dynamics for hundreds of volumes, often at subsecond intervals, at the diffraction limit and beyond. We applied this to systems spanning four orders of magnitude in space and time, including the diffusion of single transcription factor molecules in stem cell spheroids, the dynamic instability of mitotic microtubules, the immunological synapse, neutrophil motility in a 3D matrix, and embryogenesis in Caenorhabditis elegans and Drosophila melanogaster. The results provide a visceral reminder of the beauty and the complexity of living systems.
Full Text
Duke Authors
Cited Authors
- Chen, B-C; Legant, WR; Wang, K; Shao, L; Milkie, DE; Davidson, MW; Janetopoulos, C; Wu, XS; Hammer, JA; Liu, Z; English, BP; Mimori-Kiyosue, Y; Romero, DP; Ritter, AT; Lippincott-Schwartz, J; Fritz-Laylin, L; Mullins, RD; Mitchell, DM; Bembenek, JN; Reymann, A-C; Böhme, R; Grill, SW; Wang, JT; Seydoux, G; Tulu, US; Kiehart, DP; Betzig, E
Published Date
- October 23, 2014
Published In
Volume / Issue
- 346 / 6208
Start / End Page
- 1257998 -
PubMed ID
- 25342811
Pubmed Central ID
- 25342811
Electronic International Standard Serial Number (EISSN)
- 1095-9203
International Standard Serial Number (ISSN)
- 0036-8075
Digital Object Identifier (DOI)
- 10.1126/science.1257998
Language
- eng