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Murine granulocyte-macrophage colony-stimulating factor expressed from a bicistronic simian immunodeficiency virus-based integrase-defective lentiviral vector does not enhance T-cell responses in mice.

Publication ,  Journal Article
Michelini, Z; Negri, D; Biava, M; Baroncelli, S; Spada, M; Leone, P; Bona, R; Blasi, M; Nègre, D; Klotman, ME; Cara, A
Published in: Viral Immunol
December 2014

As a prelude to immunization studies in nonhuman primates, we compared in mice the immunogenicity of a simian immunodeficiency virus (SIV)-based integrase (IN)-defective lentiviral vector (IDLV) encoding the model antigen-enhanced green fluorescence protein (eGFP) in the presence or absence of the murine granulocyte-macrophage colony-stimulating factor (mGM-CSF) expressed from an internal ribosomal entry site (IRES) sequence. BALB/c mice were immunized once intramuscularly with IDLV expressing eGFP alone or eGFP and mGM-CSF and immune responses were evaluated up to 90 days from the single intramuscular immunization. Results indicated that the mGM-CSF was unable to improve the magnitude and quality of the immune response against the eGFP transgene in the context of the SIV-based IDLV, as evaluated by enzyme-linked immunosorbent spot (ELISPOT) assays for interferon-γ (IFN-γ) and by intracellular cytokine staining for IFN-γ, interleukin-2 (IL-2), and tumor necrosis factor-alpha (TNF-α). These findings suggest that for vaccination purposes, the presence of mGM-CSF expressed after the IRES in a SIV-based IDLV system does not favor the improvement of the immunological response against the transgene of interest. Further studies should investigate whether the selection of a different cytokine gene might improve the immune response against the transgene.

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Published In

Viral Immunol

DOI

EISSN

1557-8976

Publication Date

December 2014

Volume

27

Issue

10

Start / End Page

512 / 520

Location

United States

Related Subject Headings

  • Virology
  • Viral Vaccines
  • Vaccines, Synthetic
  • Tumor Necrosis Factor-alpha
  • T-Lymphocytes
  • Staining and Labeling
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Recombinant Proteins
  • Mice, Inbred BALB C
 

Citation

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Michelini, Z., Negri, D., Biava, M., Baroncelli, S., Spada, M., Leone, P., … Cara, A. (2014). Murine granulocyte-macrophage colony-stimulating factor expressed from a bicistronic simian immunodeficiency virus-based integrase-defective lentiviral vector does not enhance T-cell responses in mice. Viral Immunol, 27(10), 512–520. https://doi.org/10.1089/vim.2014.0062
Michelini, Zuleika, Donatella Negri, Mirella Biava, Silvia Baroncelli, Massimo Spada, Pasqualina Leone, Roberta Bona, et al. “Murine granulocyte-macrophage colony-stimulating factor expressed from a bicistronic simian immunodeficiency virus-based integrase-defective lentiviral vector does not enhance T-cell responses in mice.Viral Immunol 27, no. 10 (December 2014): 512–20. https://doi.org/10.1089/vim.2014.0062.
Michelini Z, Negri D, Biava M, Baroncelli S, Spada M, Leone P, Bona R, Blasi M, Nègre D, Klotman ME, Cara A. Murine granulocyte-macrophage colony-stimulating factor expressed from a bicistronic simian immunodeficiency virus-based integrase-defective lentiviral vector does not enhance T-cell responses in mice. Viral Immunol. 2014 Dec;27(10):512–520.
Journal cover image

Published In

Viral Immunol

DOI

EISSN

1557-8976

Publication Date

December 2014

Volume

27

Issue

10

Start / End Page

512 / 520

Location

United States

Related Subject Headings

  • Virology
  • Viral Vaccines
  • Vaccines, Synthetic
  • Tumor Necrosis Factor-alpha
  • T-Lymphocytes
  • Staining and Labeling
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Recombinant Proteins
  • Mice, Inbred BALB C