A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of Ranvier.

Journal Article (Journal Article)

The scaffolding protein ankyrin-G is required for Na(+) channel clustering at axon initial segments. It is also considered essential for Na(+) channel clustering at nodes of Ranvier to facilitate fast and efficient action potential propagation. However, notwithstanding these widely accepted roles, we show here that ankyrin-G is dispensable for nodal Na(+) channel clustering in vivo. Unexpectedly, in the absence of ankyrin-G, erythrocyte ankyrin (ankyrin-R) and its binding partner βI spectrin substitute for and rescue nodal Na(+) channel clustering. In addition, channel clustering is also rescued after loss of nodal βIV spectrin by βI spectrin and ankyrin-R. In mice lacking both ankyrin-G and ankyrin-R, Na(+) channels fail to cluster at nodes. Thus, ankyrin R-βI spectrin protein complexes function as secondary reserve Na(+) channel clustering machinery, and two independent ankyrin-spectrin protein complexes exist in myelinated axons to cluster Na(+) channels at nodes of Ranvier.

Full Text

Duke Authors

Cited Authors

  • Ho, TS-Y; Zollinger, DR; Chang, K-J; Xu, M; Cooper, EC; Stankewich, MC; Bennett, V; Rasband, MN

Published Date

  • December 2014

Published In

Volume / Issue

  • 17 / 12

Start / End Page

  • 1664 - 1672

PubMed ID

  • 25362473

Pubmed Central ID

  • PMC4271271

Electronic International Standard Serial Number (EISSN)

  • 1546-1726

Digital Object Identifier (DOI)

  • 10.1038/nn.3859


  • eng

Conference Location

  • United States