Glial ankyrins facilitate paranodal axoglial junction assembly.

Published

Journal Article

Neuron-glia interactions establish functional membrane domains along myelinated axons. These include nodes of Ranvier, paranodal axoglial junctions and juxtaparanodes. Paranodal junctions are the largest vertebrate junctional adhesion complex, and they are essential for rapid saltatory conduction and contribute to assembly and maintenance of nodes. However, the molecular mechanisms underlying paranodal junction assembly are poorly understood. Ankyrins are cytoskeletal scaffolds traditionally associated with Na(+) channel clustering in neurons and are important for membrane domain establishment and maintenance in many cell types. Here we show that ankyrin-B, expressed by Schwann cells, and ankyrin-G, expressed by oligodendrocytes, are highly enriched at the glial side of paranodal junctions where they interact with the essential glial junctional component neurofascin 155. Conditional knockout of ankyrins in oligodendrocytes disrupts paranodal junction assembly and delays nerve conduction during early development in mice. Thus, glial ankyrins function as major scaffolds that facilitate early and efficient paranodal junction assembly in the developing CNS.

Full Text

Duke Authors

Cited Authors

  • Chang, K-J; Zollinger, DR; Susuki, K; Sherman, DL; Makara, MA; Brophy, PJ; Cooper, EC; Bennett, V; Mohler, PJ; Rasband, MN

Published Date

  • December 2014

Published In

Volume / Issue

  • 17 / 12

Start / End Page

  • 1673 - 1681

PubMed ID

  • 25362471

Pubmed Central ID

  • 25362471

Electronic International Standard Serial Number (EISSN)

  • 1546-1726

Digital Object Identifier (DOI)

  • 10.1038/nn.3858

Language

  • eng

Conference Location

  • United States