Skip to main content

Genome accessibility is widely preserved and locally modulated during mitosis.

Publication ,  Journal Article
Hsiung, CC-S; Morrissey, CS; Udugama, M; Frank, CL; Keller, CA; Baek, S; Giardine, B; Crawford, GE; Sung, M-H; Hardison, RC; Blobel, GA
Published in: Genome Res
February 2015

Mitosis entails global alterations to chromosome structure and nuclear architecture, concomitant with transient silencing of transcription. How cells transmit transcriptional states through mitosis remains incompletely understood. While many nuclear factors dissociate from mitotic chromosomes, the observation that certain nuclear factors and chromatin features remain associated with individual loci during mitosis originated the hypothesis that such mitotically retained molecular signatures could provide transcriptional memory through mitosis. To understand the role of chromatin structure in mitotic memory, we performed the first genome-wide comparison of DNase I sensitivity of chromatin in mitosis and interphase, using a murine erythroblast model. Despite chromosome condensation during mitosis visible by microscopy, the landscape of chromatin accessibility at the macromolecular level is largely unaltered. However, mitotic chromatin accessibility is locally dynamic, with individual loci maintaining none, some, or all of their interphase accessibility. Mitotic reduction in accessibility occurs primarily within narrow, highly DNase hypersensitive sites that frequently coincide with transcription factor binding sites, whereas broader domains of moderate accessibility tend to be more stable. In mitosis, proximal promoters generally maintain their accessibility more strongly, whereas distal regulatory elements tend to lose accessibility. Large domains of DNA hypomethylation mark a subset of promoters that retain accessibility during mitosis and across many cell types in interphase. Erythroid transcription factor GATA1 exerts site-specific changes in interphase accessibility that are most pronounced at distal regulatory elements, but has little influence on mitotic accessibility. We conclude that features of open chromatin are remarkably stable through mitosis, but are modulated at the level of individual genes and regulatory elements.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Genome Res

DOI

EISSN

1549-5469

Publication Date

February 2015

Volume

25

Issue

2

Start / End Page

213 / 225

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Regulatory Sequences, Nucleic Acid
  • Protein Binding
  • Promoter Regions, Genetic
  • Mitosis
  • Mice
  • Interphase
  • High-Throughput Nucleotide Sequencing
  • Genome
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hsiung, C.-S., Morrissey, C. S., Udugama, M., Frank, C. L., Keller, C. A., Baek, S., … Blobel, G. A. (2015). Genome accessibility is widely preserved and locally modulated during mitosis. Genome Res, 25(2), 213–225. https://doi.org/10.1101/gr.180646.114
Hsiung, Chris C-S, Christapher S. Morrissey, Maheshi Udugama, Christopher L. Frank, Cheryl A. Keller, Songjoon Baek, Belinda Giardine, et al. “Genome accessibility is widely preserved and locally modulated during mitosis.Genome Res 25, no. 2 (February 2015): 213–25. https://doi.org/10.1101/gr.180646.114.
Hsiung CC-S, Morrissey CS, Udugama M, Frank CL, Keller CA, Baek S, et al. Genome accessibility is widely preserved and locally modulated during mitosis. Genome Res. 2015 Feb;25(2):213–25.
Hsiung, Chris C. S., et al. “Genome accessibility is widely preserved and locally modulated during mitosis.Genome Res, vol. 25, no. 2, Feb. 2015, pp. 213–25. Pubmed, doi:10.1101/gr.180646.114.
Hsiung CC-S, Morrissey CS, Udugama M, Frank CL, Keller CA, Baek S, Giardine B, Crawford GE, Sung M-H, Hardison RC, Blobel GA. Genome accessibility is widely preserved and locally modulated during mitosis. Genome Res. 2015 Feb;25(2):213–225.

Published In

Genome Res

DOI

EISSN

1549-5469

Publication Date

February 2015

Volume

25

Issue

2

Start / End Page

213 / 225

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Regulatory Sequences, Nucleic Acid
  • Protein Binding
  • Promoter Regions, Genetic
  • Mitosis
  • Mice
  • Interphase
  • High-Throughput Nucleotide Sequencing
  • Genome