Chromosome rearrangements via template switching between diverged repeated sequences.
Journal Article
Recent high-resolution genome analyses of cancer and other diseases have revealed the occurrence of microhomology-mediated chromosome rearrangements and copy number changes. Although some of these rearrangements appear to involve nonhomologous end-joining, many must have involved mechanisms requiring new DNA synthesis. Models such as microhomology-mediated break-induced replication (MM-BIR) have been invoked to explain these rearrangements. We examined BIR and template switching between highly diverged sequences in Saccharomyces cerevisiae, induced during repair of a site-specific double-strand break (DSB). Our data show that such template switches are robust mechanisms that give rise to complex rearrangements. Template switches between highly divergent sequences appear to be mechanistically distinct from the initial strand invasions that establish BIR. In particular, such jumps are less constrained by sequence divergence and exhibit a different pattern of microhomology junctions. BIR traversing repeated DNA sequences frequently results in complex translocations analogous to those seen in mammalian cells. These results suggest that template switching among repeated genes is a potent driver of genome instability and evolution.
Full Text
Duke Authors
Cited Authors
- Anand, RP; Tsaponina, O; Greenwell, PW; Lee, C-S; Du, W; Petes, TD; Haber, JE
Published Date
- November 1, 2014
Published In
Volume / Issue
- 28 / 21
Start / End Page
- 2394 - 2406
PubMed ID
- 25367035
Pubmed Central ID
- 25367035
Electronic International Standard Serial Number (EISSN)
- 1549-5477
Digital Object Identifier (DOI)
- 10.1101/gad.250258.114
Language
- eng
Conference Location
- United States