Femtosecond pulse train shaping improves two-photon excited fluorescence measurements.


Journal Article

Measurements of two-photon absorption (TPA) cross sections are greatly confounded by even very weak linear absorption, for example from hot bands. In this case, the experimental power dependence of fluorescence from amplified and mode-locked laser systems can differ drastically, even if the peak intensity is adjusted to be the same in both cases. A simple pulse train shaping method suppresses linear contributions and extracts the nonlinear absorption cross section, demonstrated here for a meso-to-meso ethyne-bridged bis[(porphinato)zinc(II)] fluorophore (DD) at 800 nm. This approach permits reliable TPA cross-section measurement, even with standard modelocked lasers under conditions identical to that used for multiphoton microscopy.

Full Text

Duke Authors

Cited Authors

  • Park, JK; Fischer, MC; Susumu, K; Therien, MJ; Warren, WS

Published Date

  • October 2014

Published In

Volume / Issue

  • 39 / 19

Start / End Page

  • 5606 - 5609

PubMed ID

  • 25360939

Pubmed Central ID

  • 25360939

Electronic International Standard Serial Number (EISSN)

  • 1539-4794

International Standard Serial Number (ISSN)

  • 0146-9592

Digital Object Identifier (DOI)

  • 10.1364/ol.39.005606


  • eng