Wavelet transform fast inverse light scattering analysis for size determination of spherical scatterers.


Journal Article

We present a fast approach for size determination of spherical scatterers using the continuous wavelet transform of the angular light scattering profile to address the computational limitations of previously developed sizing techniques. The potential accuracy, speed, and robustness of the algorithm were determined in simulated models of scattering by polystyrene beads and cells. The algorithm was tested experimentally on angular light scattering data from polystyrene bead phantoms and MCF-7 breast cancer cells using a 2D a/LCI system. Theoretical sizing of simulated profiles of beads and cells produced strong fits between calculated and actual size (r(2) = 0.9969 and r(2) = 0.9979 respectively), and experimental size determinations were accurate to within one micron.

Full Text

Duke Authors

Cited Authors

  • Ho, D; Kim, S; Drake, TK; Eldridge, WJ; Wax, A

Published Date

  • October 2014

Published In

Volume / Issue

  • 5 / 10

Start / End Page

  • 3292 - 3304

PubMed ID

  • 25360350

Pubmed Central ID

  • 25360350

Electronic International Standard Serial Number (EISSN)

  • 2156-7085

International Standard Serial Number (ISSN)

  • 2156-7085

Digital Object Identifier (DOI)

  • 10.1364/boe.5.003292


  • eng