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c-FLIP protects eosinophils from TNF-α-mediated cell death in vivo.

Publication ,  Journal Article
Gordy, C; Liang, J; Pua, H; He, Y-W
Published in: PLoS One
2014

Understanding the signals that regulate eosinophil survival and death is critical to developing new treatments for asthma, atopy, and gastrointestinal disease. Previous studies suggest that TNF-α stimulation protects eosinophils from apoptosis, and this TNF-α-mediated protection is mediated by the upregulation of an unknown protein by NF-κB. Here, we show for the first time that eosinophils express the caspase 8-inhibitory protein c-FLIP, and c-FLIP expression is upregulated upon TNF-α stimulation. Considering that c-FLIP expression is regulated by NF-κB, we hypothesized that c-FLIP might serve as the "molecular switch" that converts TNFRI activation to a pro-survival signal in eosinophils. Indeed, we found that one c-FLIP isoform, c-FLIPL, is required for mouse eosinophil survival in the presence of TNF-α both in vitro and in vivo. Importantly, our results suggest c-FLIP as a potential therapeutic target for the treatment of eosinophil-mediated disease.

Duke Scholars

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2014

Volume

9

Issue

10

Start / End Page

e107724

Location

United States

Related Subject Headings

  • Up-Regulation
  • Tumor Necrosis Factor-alpha
  • NF-kappa B
  • Mice, Transgenic
  • Mice
  • Genotype
  • General Science & Technology
  • Eosinophils
  • Chromosomes, Artificial, Bacterial
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Gordy, C., Liang, J., Pua, H., & He, Y.-W. (2014). c-FLIP protects eosinophils from TNF-α-mediated cell death in vivo. PLoS One, 9(10), e107724. https://doi.org/10.1371/journal.pone.0107724
Gordy, Claire, Jie Liang, Heather Pua, and You-Wen He. “c-FLIP protects eosinophils from TNF-α-mediated cell death in vivo.PLoS One 9, no. 10 (2014): e107724. https://doi.org/10.1371/journal.pone.0107724.
Gordy C, Liang J, Pua H, He Y-W. c-FLIP protects eosinophils from TNF-α-mediated cell death in vivo. PLoS One. 2014;9(10):e107724.
Gordy, Claire, et al. “c-FLIP protects eosinophils from TNF-α-mediated cell death in vivo.PLoS One, vol. 9, no. 10, 2014, p. e107724. Pubmed, doi:10.1371/journal.pone.0107724.
Gordy C, Liang J, Pua H, He Y-W. c-FLIP protects eosinophils from TNF-α-mediated cell death in vivo. PLoS One. 2014;9(10):e107724.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2014

Volume

9

Issue

10

Start / End Page

e107724

Location

United States

Related Subject Headings

  • Up-Regulation
  • Tumor Necrosis Factor-alpha
  • NF-kappa B
  • Mice, Transgenic
  • Mice
  • Genotype
  • General Science & Technology
  • Eosinophils
  • Chromosomes, Artificial, Bacterial
  • Cells, Cultured