Synaptic clustering of AMPA receptors by the extracellular immediate-early gene product Narp.

Published

Journal Article

Narp (neuronal activity-regulated pentraxin) is a secreted immediate-early gene (IEG) regulated by synaptic activity in brain. In this study, we demonstrate that Narp possesses several properties that make it likely to play a key role in excitatory synaptogenesis. Narp is shown to be selectively enriched at excitatory synapses on neurons from both the hippocampus and spinal cord. Overexpression of recombinant Narp increases the number of excitatory but not inhibitory synapses in cultured spinal neurons. In transfected HEK 293T cells, Narp interacts with itself, forming large surface clusters that coaggregate AMPA receptor subunits. Moreover, Narp-expressing HEK 293T cells can induce the aggregation of neuronal AMPA receptors. These studies support a model in which Narp functions as an extracellular aggregating factor for AMPA receptors.

Full Text

Duke Authors

Cited Authors

  • O'Brien, RJ; Xu, D; Petralia, RS; Steward, O; Huganir, RL; Worley, P

Published Date

  • June 1999

Published In

Volume / Issue

  • 23 / 2

Start / End Page

  • 309 - 323

PubMed ID

  • 10399937

Pubmed Central ID

  • 10399937

Electronic International Standard Serial Number (EISSN)

  • 1097-4199

International Standard Serial Number (ISSN)

  • 0896-6273

Digital Object Identifier (DOI)

  • 10.1016/s0896-6273(00)80782-5

Language

  • eng