Automated measurement of microcirculatory blood flow velocity in pulmonary metastases of rats.

Published online

Journal Article

Because the lung is a major target organ of metastatic disease, animal models to study the physiology of pulmonary metastases are of great importance. However, very few methods exist to date to investigate lung metastases in a dynamic fashion at the microcirculatory level, due to the difficulty to access the lung with a microscope. Here, an intravital microscopy method is presented to functionally image and quantify the microcirculation of superficial pulmonary metastases in rats, using a closed-chest pulmonary window and automated analysis of blood flow velocity and direction. The utility of this method is demonstrated to measure increases in blood flow velocity in response to pharmacological intervention, and to image the well-known tortuous vasculature of solid tumors. This is the first demonstration of intravital microscopy on pulmonary metastases in a closed-chest model. Because of its minimized invasiveness, as well as due to its relative ease and practicality, this technology has the potential to experience widespread use in laboratories that specialize on pulmonary tumor research.

Full Text

Duke Authors

Cited Authors

  • Blueschke, G; Hanna, G; Fontanella, AN; Palmer, GM; Boico, A; Min, H; Dewhirst, MW; Irwin, DC; Zhao, Y; Schroeder, T

Published Date

  • November 30, 2014

Published In

Start / End Page

  • e51630 -

PubMed ID

  • 25490280

Pubmed Central ID

  • 25490280

Electronic International Standard Serial Number (EISSN)

  • 1940-087X

Digital Object Identifier (DOI)

  • 10.3791/51630

Language

  • eng

Conference Location

  • United States