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A CRISPR/Cas9-based system for reprogramming cell lineage specification.

Publication ,  Journal Article
Chakraborty, S; Ji, H; Kabadi, AM; Gersbach, CA; Christoforou, N; Leong, KW
Published in: Stem cell reports
December 2014

Gene activation by the CRISPR/Cas9 system has the potential to enable new approaches to science and medicine, but the technology must be enhanced to robustly control cell behavior. We show that the fusion of two transactivation domains to Cas9 dramatically enhances gene activation to a level that is necessary to reprogram cell phenotype. Targeted activation of the endogenous Myod1 gene locus with this system led to stable and sustained reprogramming of mouse embryonic fibroblasts into skeletal myocytes. The levels of myogenic marker expression obtained by the activation of endogenous Myod1 gene were comparable to that achieved by overexpression of lentivirally delivered MYOD1 transcription factor.

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Published In

Stem cell reports

DOI

EISSN

2213-6711

ISSN

2213-6711

Publication Date

December 2014

Volume

3

Issue

6

Start / End Page

940 / 947

Related Subject Headings

  • Recombinant Fusion Proteins
  • Organ Specificity
  • MyoD Protein
  • Mice
  • Gene Expression Regulation
  • Gene Expression
  • Cellular Reprogramming
  • Cell Lineage
  • Cell Line
  • CRISPR-Cas Systems
 

Citation

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Chakraborty, S., Ji, H., Kabadi, A. M., Gersbach, C. A., Christoforou, N., & Leong, K. W. (2014). A CRISPR/Cas9-based system for reprogramming cell lineage specification. Stem Cell Reports, 3(6), 940–947. https://doi.org/10.1016/j.stemcr.2014.09.013
Chakraborty, Syandan, HaYeun Ji, Ami M. Kabadi, Charles A. Gersbach, Nicolas Christoforou, and Kam W. Leong. “A CRISPR/Cas9-based system for reprogramming cell lineage specification.Stem Cell Reports 3, no. 6 (December 2014): 940–47. https://doi.org/10.1016/j.stemcr.2014.09.013.
Chakraborty S, Ji H, Kabadi AM, Gersbach CA, Christoforou N, Leong KW. A CRISPR/Cas9-based system for reprogramming cell lineage specification. Stem cell reports. 2014 Dec;3(6):940–7.
Chakraborty, Syandan, et al. “A CRISPR/Cas9-based system for reprogramming cell lineage specification.Stem Cell Reports, vol. 3, no. 6, Dec. 2014, pp. 940–47. Epmc, doi:10.1016/j.stemcr.2014.09.013.
Chakraborty S, Ji H, Kabadi AM, Gersbach CA, Christoforou N, Leong KW. A CRISPR/Cas9-based system for reprogramming cell lineage specification. Stem cell reports. 2014 Dec;3(6):940–947.
Journal cover image

Published In

Stem cell reports

DOI

EISSN

2213-6711

ISSN

2213-6711

Publication Date

December 2014

Volume

3

Issue

6

Start / End Page

940 / 947

Related Subject Headings

  • Recombinant Fusion Proteins
  • Organ Specificity
  • MyoD Protein
  • Mice
  • Gene Expression Regulation
  • Gene Expression
  • Cellular Reprogramming
  • Cell Lineage
  • Cell Line
  • CRISPR-Cas Systems