Improving the odorant sensitivity of olfactory receptor-expressing yeast with accessory proteins.

Published

Journal Article

Olfaction depends on the selectivity and sensitivity of olfactory receptors. Previous attempts at constructing a mammalian olfactory receptor-based artificial odorant sensing system in the budding yeast Saccharomyces cerevisiae suffered from low sensitivity and activity. This result may be at least in part due to poor functional expression of olfactory receptors and/or limited solubility of some odorants in the medium. In this study, we examined the effects of two types of accessory proteins, receptor transporting protein 1 short and odorant binding proteins, in improving odor-mediated activation of olfactory receptors expressed in yeast. We found that receptor transporting protein 1 short enhanced the membrane expression and ligand-induced responses of some olfactory receptors. Coexpression of odorant binding proteins of the silkworm moth Bombyx mori enhanced the sensitivity of a mouse olfactory receptor. Our results suggest that different classes of accessory proteins can confer sensitive and robust responses of olfactory receptors expressed in yeast. Inclusion of accessory proteins may be essential in the future development of practical olfactory receptor-based odorant sensors.

Full Text

Duke Authors

Cited Authors

  • Fukutani, Y; Hori, A; Tsukada, S; Sato, R; Ishii, J; Kondo, A; Matsunami, H; Yohda, M

Published Date

  • February 15, 2015

Published In

Volume / Issue

  • 471 /

Start / End Page

  • 1 - 8

PubMed ID

  • 25449303

Pubmed Central ID

  • 25449303

Electronic International Standard Serial Number (EISSN)

  • 1096-0309

Digital Object Identifier (DOI)

  • 10.1016/j.ab.2014.10.012

Language

  • eng

Conference Location

  • United States