Developmental mechanism of the periodic membrane skeleton in axons.

Journal Article (Journal Article)

Actin, spectrin, and associated molecules form a periodic sub-membrane lattice structure in axons. How this membrane skeleton is developed and why it preferentially forms in axons are unknown. Here, we studied the developmental mechanism of this lattice structure. We found that this structure emerged early during axon development and propagated from proximal regions to distal ends of axons. Components of the axon initial segment were recruited to the lattice late during development. Formation of the lattice was regulated by the local concentration of βII spectrin, which is higher in axons than in dendrites. Increasing the dendritic concentration of βII spectrin by overexpression or by knocking out ankyrin B induced the formation of the periodic structure in dendrites, demonstrating that the spectrin concentration is a key determinant in the preferential development of this structure in axons and that ankyrin B is critical for the polarized distribution of βII spectrin in neurites.

Full Text

Duke Authors

Cited Authors

  • Zhong, G; He, J; Zhou, R; Lorenzo, D; Babcock, HP; Bennett, V; Zhuang, X

Published Date

  • December 23, 2014

Published In

Volume / Issue

  • 3 /

PubMed ID

  • 25535840

Pubmed Central ID

  • PMC4337613

Electronic International Standard Serial Number (EISSN)

  • 2050-084X

Digital Object Identifier (DOI)

  • 10.7554/eLife.04581

Language

  • eng

Conference Location

  • England