Magnetic resonance imaging of graded skeletal muscle injury in live rats.

Published online

Journal Article

INTRODUCTION: Increasing number of stretch-shortening contractions (SSCs) results in increased muscle injury. METHODS: Fischer Hybrid rats were acutely exposed to an increasing number of SSCs in vivo using a custom-designed dynamometer. Magnetic resonance imaging (MRI) imaging was conducted 72 hours after exposure when rats were infused with Prohance and imaged using a 7T rodent MRI system (GE Epic 12.0). Images were acquired in the transverse plane with typically 60 total slices acquired covering the entire length of the hind legs. Rats were euthanized after MRI, the lower limbs removed, and tibialis anterior muscles were prepared for histology and quantified stereology. RESULTS: Stereological analyses showed myofiber degeneration, and cellular infiltrates significantly increased following 70 and 150 SSC exposure compared to controls. MRI images revealed that the percent affected area significantly increased with exposure in all SSC groups in a graded fashion. Signal intensity also significantly increased with increasing SSC repetitions. DISCUSSION: These results suggest that contrast-enhanced MRI has the sensitivity to differentiate specific degrees of skeletal muscle strain injury, and imaging data are specifically representative of cellular histopathology quantified via stereological analyses.

Full Text

Duke Authors

Cited Authors

  • Cutlip, RG; Hollander, MS; Johnson, GA; Johnson, BW; Friend, SA; Baker, BA

Published Date

  • 2014

Published In

Volume / Issue

  • 8 / Suppl 1

Start / End Page

  • 31 - 39

PubMed ID

  • 25525369

Pubmed Central ID

  • 25525369

International Standard Serial Number (ISSN)

  • 1178-6302

Digital Object Identifier (DOI)

  • 10.4137/EHI.S15255


  • eng

Conference Location

  • United States