Comparison of proteomic and metabolomic profiles of mutants of the mitochondrial respiratory chain in Caenorhabditis elegans.

Published

Journal Article

Single-gene mutations that disrupt mitochondrial respiratory chain function in Caenorhabditis elegans change patterns of protein expression and metabolites. Our goal was to develop useful molecular fingerprints employing adaptable techniques to recognize mitochondrial defects in the electron transport chain. We analyzed mutations affecting complex I, complex II, or ubiquinone synthesis and discovered overarching patterns in the response of C. elegans to mitochondrial dysfunction across all of the mutations studied. These patterns are in KEGG pathways conserved from C. elegans to mammals, verifying that the nematode can serve as a model for mammalian disease. In addition, specific differences exist between mutants that may be useful in diagnosing specific mitochondrial diseases in patients.

Full Text

Duke Authors

Cited Authors

  • Morgan, PG; Higdon, R; Kolker, N; Bauman, AT; Ilkayeva, O; Newgard, CB; Kolker, E; Steele, LM; Sedensky, MM

Published Date

  • January 2015

Published In

Volume / Issue

  • 20 /

Start / End Page

  • 95 - 102

PubMed ID

  • 25530493

Pubmed Central ID

  • 25530493

Electronic International Standard Serial Number (EISSN)

  • 1872-8278

Digital Object Identifier (DOI)

  • 10.1016/j.mito.2014.12.004

Language

  • eng

Conference Location

  • Netherlands