Actin cytoskeletal disruption following cryopreservation alters the biodistribution of human mesenchymal stromal cells in vivo.

Published

Journal Article

Mesenchymal stromal cells have shown clinical promise; however, variations in treatment responses are an ongoing concern. We previously demonstrated that MSCs are functionally stunned after thawing. Here, we investigated whether this cryopreservation/thawing defect also impacts the postinfusion biodistribution properties of MSCs. Under both static and physiologic flow, compared with live MSCs in active culture, MSCs thawed from cryopreservation bound poorly to fibronectin (40% reduction) and human endothelial cells (80% reduction), respectively. This reduction correlated with a reduced cytoskeletal F-actin content in post-thaw MSCs (60% reduction). In vivo, live human MSCs could be detected in murine lung tissues for up to 24 hr, whereas thawed MSCs were undetectable. Similarly, live MSCs whose actin cytoskeleton was chemically disrupted were undetectable at 24 hr postinfusion. Our data suggest that post-thaw cryopreserved MSCs are distinct from live MSCs. This distinction could significantly affect the utility of MSCs as a cellular therapeutic.

Full Text

Duke Authors

Cited Authors

  • Chinnadurai, R; Garcia, MA; Sakurai, Y; Lam, WA; Kirk, AD; Galipeau, J; Copland, IB

Published Date

  • July 2014

Published In

Volume / Issue

  • 3 / 1

Start / End Page

  • 60 - 72

PubMed ID

  • 25068122

Pubmed Central ID

  • 25068122

Electronic International Standard Serial Number (EISSN)

  • 2213-6711

International Standard Serial Number (ISSN)

  • 2213-6711

Digital Object Identifier (DOI)

  • 10.1016/j.stemcr.2014.05.003

Language

  • eng