Skip to main content

Surgical correction of gastroesophageal reflux in lung transplant patients is associated with decreased effector CD8 cells in lung lavages: a case series.

Publication ,  Journal Article
Neujahr, DC; Mohammed, A; Ulukpo, O; Force, SD; Ramirez, AM; Pelaez, A; Lawrence, EC; Larsen, CP; Kirk, AD
Published in: Chest
October 2010

BACKGROUND: Lung transplantation is associated with a high incidence of gastroesophageal reflux disease (GERD). The presence of GERD is considered a risk factor for the subsequent development of obliterative bronchiolitis (OB), and surgical correction of GERD by gastric fundoplication (GF) may be associated with increased freedom from OB. The mechanisms underlying a protective effect from OB remain elusive. The objective of this study was to analyze the flow cytometric properties of BAL cells in patients who have undergone GF early after transplant. METHODS: In a single-center lung transplant center, eight patients with GERD who were in the first transplant year underwent GF. Prior to and immediately following GF, BAL cells were analyzed by polychromatic flow cytometry. Spirometry was performed before and after GF. RESULTS: GF was associated with a significant reduction in the frequency of BAL CD8 lymphocytes expressing the intracellular effector marker granzyme B, compared with the pre-GF levels. Twenty-six percent of CD8 cells were granzyme Bhi pre-GF compared with 12% of CD8 cells post-GF (range 8%-50% pre-GF, 2%-24% post-GF, P = .01). In contrast, GF was associated with a significant interval increase in the frequency of CD8 cells with an exhausted phenotype (granzyme Blo, CD127lo, PD1hi) from 12% of CD8 cells pre-GF to 24% post-GF (range 1.7%-24% pre-GF and 11%-47% post-GF, P = .05). No significant changes in spirometry were observed during the study interval. CONCLUSIONS: Surgical correction of GF is associated with a decreased frequency of potentially injurious effector CD8 cells in the BAL of lung transplant recipients.

Duke Scholars

Published In

Chest

DOI

EISSN

1931-3543

Publication Date

October 2010

Volume

138

Issue

4

Start / End Page

937 / 943

Location

United States

Related Subject Headings

  • Spirometry
  • Respiratory System
  • Postoperative Complications
  • Middle Aged
  • Male
  • Lung Transplantation
  • Humans
  • Granzymes
  • Gastroesophageal Reflux
  • Flow Cytometry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Neujahr, D. C., Mohammed, A., Ulukpo, O., Force, S. D., Ramirez, A. M., Pelaez, A., … Kirk, A. D. (2010). Surgical correction of gastroesophageal reflux in lung transplant patients is associated with decreased effector CD8 cells in lung lavages: a case series. Chest, 138(4), 937–943. https://doi.org/10.1378/chest.09-2888
Neujahr, David C., Aminu Mohammed, Onome Ulukpo, Seth D. Force, Allan M. Ramirez, Andres Pelaez, E Clinton Lawrence, Christian P. Larsen, and Allan D. Kirk. “Surgical correction of gastroesophageal reflux in lung transplant patients is associated with decreased effector CD8 cells in lung lavages: a case series.Chest 138, no. 4 (October 2010): 937–43. https://doi.org/10.1378/chest.09-2888.
Neujahr DC, Mohammed A, Ulukpo O, Force SD, Ramirez AM, Pelaez A, et al. Surgical correction of gastroesophageal reflux in lung transplant patients is associated with decreased effector CD8 cells in lung lavages: a case series. Chest. 2010 Oct;138(4):937–43.
Neujahr, David C., et al. “Surgical correction of gastroesophageal reflux in lung transplant patients is associated with decreased effector CD8 cells in lung lavages: a case series.Chest, vol. 138, no. 4, Oct. 2010, pp. 937–43. Pubmed, doi:10.1378/chest.09-2888.
Neujahr DC, Mohammed A, Ulukpo O, Force SD, Ramirez AM, Pelaez A, Lawrence EC, Larsen CP, Kirk AD. Surgical correction of gastroesophageal reflux in lung transplant patients is associated with decreased effector CD8 cells in lung lavages: a case series. Chest. 2010 Oct;138(4):937–943.

Published In

Chest

DOI

EISSN

1931-3543

Publication Date

October 2010

Volume

138

Issue

4

Start / End Page

937 / 943

Location

United States

Related Subject Headings

  • Spirometry
  • Respiratory System
  • Postoperative Complications
  • Middle Aged
  • Male
  • Lung Transplantation
  • Humans
  • Granzymes
  • Gastroesophageal Reflux
  • Flow Cytometry