Skip to main content

Induction of specific tolerance by intrathymic injection of recipient muscle cells transfected with donor class I major histocompatibility complex.

Publication ,  Journal Article
Knechtle, SJ; Wang, J; Jiao, S; Geissler, EK; Sumimoto, R; Wolff, J
Published in: Transplantation
April 15, 1994

Induction of tolerance to allogeneic MHC antigens has been a goal in the field of transplantation because it would reduce or eliminate the need for generalized immunosuppression. Although encouraging results have been obtained in experimental models by exposing recipient thymus to donor cells before transplantation, donor cells are not typically available at that time, and the donor antigens responsible for the effect are poorly defined. In the present study, thymic tolerance was demonstrated without using donor cells. Recipient thymus was injected before transplantation with autologous myoblasts and myotubes that were genetically modified to express allogeneic donor-type MHC class I antigen. Donor-specific unresponsiveness was induced to a completely MHC-disparate liver transplant and to a subsequent donor-type cardiac allograft, but not a third-party allograft. In vitro, recipient CTL demonstrated a 10-fold reduction in killing of donor cells, but not of third-party cells. Our results demonstrate: (1) that recipient muscle cells can be genetically engineered to induce donor-specific unresponsiveness when given intrathymically, and (2) transfected recipient cells expressing only donor MHC class I antigen can induce tolerance to a fully allogeneic donor.

Duke Scholars

Published In

Transplantation

ISSN

0041-1337

Publication Date

April 15, 1994

Volume

57

Issue

7

Start / End Page

990 / 996

Location

United States

Related Subject Headings

  • Transfection
  • Thymus Gland
  • T-Lymphocytes, Cytotoxic
  • Surgery
  • Rats, Inbred Lew
  • Rats, Inbred ACI
  • Rats
  • Muscles
  • Male
  • Liver Transplantation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Knechtle, S. J., Wang, J., Jiao, S., Geissler, E. K., Sumimoto, R., & Wolff, J. (1994). Induction of specific tolerance by intrathymic injection of recipient muscle cells transfected with donor class I major histocompatibility complex. Transplantation, 57(7), 990–996.
Knechtle, S. J., J. Wang, S. Jiao, E. K. Geissler, R. Sumimoto, and J. Wolff. “Induction of specific tolerance by intrathymic injection of recipient muscle cells transfected with donor class I major histocompatibility complex.Transplantation 57, no. 7 (April 15, 1994): 990–96.
Knechtle SJ, Wang J, Jiao S, Geissler EK, Sumimoto R, Wolff J. Induction of specific tolerance by intrathymic injection of recipient muscle cells transfected with donor class I major histocompatibility complex. Transplantation. 1994 Apr 15;57(7):990–6.
Knechtle SJ, Wang J, Jiao S, Geissler EK, Sumimoto R, Wolff J. Induction of specific tolerance by intrathymic injection of recipient muscle cells transfected with donor class I major histocompatibility complex. Transplantation. 1994 Apr 15;57(7):990–996.

Published In

Transplantation

ISSN

0041-1337

Publication Date

April 15, 1994

Volume

57

Issue

7

Start / End Page

990 / 996

Location

United States

Related Subject Headings

  • Transfection
  • Thymus Gland
  • T-Lymphocytes, Cytotoxic
  • Surgery
  • Rats, Inbred Lew
  • Rats, Inbred ACI
  • Rats
  • Muscles
  • Male
  • Liver Transplantation