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Identification of bacterial antigens in circulating immune complexes of infective endocarditis.

Publication ,  Journal Article
Inman, RD; Redecha, PB; Knechtle, SJ; Schned, ES; van de Rijn, I; Christian, CL
Published in: J Clin Invest
August 1982

The presence of circulating immune complexes (IC) in patients with infective endocarditis has been well documented but the contributions of host and bacterial components to these IC have not been defined. To study this question, IC were isolated from serum of a patient with Streptococcus faecalis endocarditis by differential polyethylene glycol precipitation and competitive binding to staphylococcal protein A. A rabbit antiserum raised against the purified IC had reactivity by crossed immunoelectrophoresis primarily with an antigen derived from the cytoplasm of the infective organism. The antigen was a protein with a 12,000-dalton molecular mass. In situ radiolabeling of the IC bound to the protein A demonstrated a component of the same molecular mass as the bacterial antigen recognized by the antiserum. The patient serum had multiple antibody specificities reactive with bacterial antigens, including the antigen recognized by the rabbit anti-IC antiserum. These techniques for isolation and characterization of circulating IC may have value in the study of IC diseases in which the inciting antigens are not known.

Duke Scholars

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

August 1982

Volume

70

Issue

2

Start / End Page

271 / 280

Location

United States

Related Subject Headings

  • Streptococcal Infections
  • Staphylococcal Protein A
  • Rabbits
  • Molecular Weight
  • Immunology
  • Immunoelectrophoresis, Two-Dimensional
  • Humans
  • Enterococcus faecalis
  • Endocarditis, Bacterial
  • Electrophoresis, Polyacrylamide Gel
 

Citation

APA
Chicago
ICMJE
MLA
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Inman, R. D., Redecha, P. B., Knechtle, S. J., Schned, E. S., van de Rijn, I., & Christian, C. L. (1982). Identification of bacterial antigens in circulating immune complexes of infective endocarditis. J Clin Invest, 70(2), 271–280. https://doi.org/10.1172/jci110614
Inman, R. D., P. B. Redecha, S. J. Knechtle, E. S. Schned, I. van de Rijn, and C. L. Christian. “Identification of bacterial antigens in circulating immune complexes of infective endocarditis.J Clin Invest 70, no. 2 (August 1982): 271–80. https://doi.org/10.1172/jci110614.
Inman RD, Redecha PB, Knechtle SJ, Schned ES, van de Rijn I, Christian CL. Identification of bacterial antigens in circulating immune complexes of infective endocarditis. J Clin Invest. 1982 Aug;70(2):271–80.
Inman, R. D., et al. “Identification of bacterial antigens in circulating immune complexes of infective endocarditis.J Clin Invest, vol. 70, no. 2, Aug. 1982, pp. 271–80. Pubmed, doi:10.1172/jci110614.
Inman RD, Redecha PB, Knechtle SJ, Schned ES, van de Rijn I, Christian CL. Identification of bacterial antigens in circulating immune complexes of infective endocarditis. J Clin Invest. 1982 Aug;70(2):271–280.

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

August 1982

Volume

70

Issue

2

Start / End Page

271 / 280

Location

United States

Related Subject Headings

  • Streptococcal Infections
  • Staphylococcal Protein A
  • Rabbits
  • Molecular Weight
  • Immunology
  • Immunoelectrophoresis, Two-Dimensional
  • Humans
  • Enterococcus faecalis
  • Endocarditis, Bacterial
  • Electrophoresis, Polyacrylamide Gel