The effects of hypochlorous acid and neutrophil proteases on the structure and function of extracellular superoxide dismutase.

Published

Journal Article

Extracellular superoxide dismutase (EC-SOD) is expressed by both macrophages and neutrophils and is known to influence the inflammatory response. Upon activation, neutrophils generate hypochlorous acid (HOCl) and secrete proteases to combat invading microorganisms. This produces a hostile environment in which enzymatic activity in general is challenged. In this study, we show that EC-SOD exposed to physiologically relevant concentrations of HOCl remains enzymatically active and retains the heparin-binding capacity, although HOCl exposure established oxidative modification of the N-terminal region (Met32) and the formation of an intermolecular cross-link in a fraction of the molecules. The cross-linking was also induced by activated neutrophils. Moreover, we show that the neutrophil-derived proteases human neutrophil elastase and cathepsin G cleaved the N-terminal region of EC-SOD irrespective of HOCl oxidation. Although the cleavage by elastase did not affect the quaternary structure, the cleavage by cathepsin G dissociated the molecule to produce EC-SOD monomers. The present data suggest that EC-SOD is stable and active at the site of inflammation and that neutrophils have the capacity to modulate the biodistribution of the protein by generating EC-SOD monomers that can diffuse into tissue.

Full Text

Cited Authors

  • Morales, K; Olesen, MN; Poulsen, ET; Larsen, UG; Enghild, JJ; Petersen, SV

Published Date

  • April 2015

Published In

Volume / Issue

  • 81 /

Start / End Page

  • 38 - 46

PubMed ID

  • 25582887

Pubmed Central ID

  • 25582887

Electronic International Standard Serial Number (EISSN)

  • 1873-4596

International Standard Serial Number (ISSN)

  • 0891-5849

Digital Object Identifier (DOI)

  • 10.1016/j.freeradbiomed.2014.12.027

Language

  • eng