Progenitor cell mobilization enhances bone healing by means of improved neovascularization and osteogenesis.

Published

Journal Article

BACKGROUND: Although bone repair is a relatively efficient process, a significant portion of patients fail to heal their fractures. Because adequate blood supply is essential to osteogenesis, the authors hypothesize that augmenting neovascularization by increasing the number of circulating progenitor cells will improve bony healing. METHODS: Bilateral full-thickness defects were created in the parietal bones of C57 wild-type mice. Intraperitoneal AMD3100 (n = 33) or sterile saline (n = 33) was administered daily beginning on postoperative day 3 and continuing through day 18. Circulating progenitor cell number was quantified by fluorescence-activated cell sorting. Bone regeneration was assessed with micro-computed tomography. Immunofluorescent CD31 and osteocalcin staining was performed to assess for vascularity and osteoblast density. RESULTS: AMD3100 treatment increased circulating progenitor cell levels and significantly improved bone regeneration. Calvarial defects of AMD3100-treated mice demonstrated increased vascularity and osteoblast density. CONCLUSIONS: Improved bone regeneration in this model was associated with elevated circulating progenitor cell number and subsequently improved neovascularization and osteogenesis. These findings highlight the importance of circulating progenitor cells in bone healing and may provide a novel therapy for bone regeneration.

Full Text

Duke Authors

Cited Authors

  • Wang, XX; Allen, RJ; Tutela, JP; Sailon, A; Allori, AC; Davidson, EH; Paek, GK; Saadeh, PB; McCarthy, JG; Warren, SM

Published Date

  • August 2011

Published In

Volume / Issue

  • 128 / 2

Start / End Page

  • 395 - 405

PubMed ID

  • 21788831

Pubmed Central ID

  • 21788831

Electronic International Standard Serial Number (EISSN)

  • 1529-4242

Digital Object Identifier (DOI)

  • 10.1097/PRS.0b013e31821e6e10

Language

  • eng

Conference Location

  • United States