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Scaffold-based rhBMP-2 therapy in a rat alveolar defect model: implications for human gingivoperiosteoplasty.

Publication ,  Journal Article
Nguyen, PD; Lin, CD; Allori, AC; Schachar, JS; Ricci, JL; Saadeh, PB; Warren, SM
Published in: Plast Reconstr Surg
December 2009

BACKGROUND: Primary alveolar cleft repair has a 41 to 73 percent success rate. Patients with persistent alveolar defects require secondary bone grafting. The authors investigated scaffold-based therapies designed to augment the success of alveolar repair. METHODS: Critical-size, 7 x 4 x 3-mm alveolar defects were created surgically in 60 Sprague-Dawley rats. Four scaffold treatment arms were tested: absorbable collagen sponge, absorbable collagen sponge plus recombinant human bone morphogenetic protein-2 (rhBMP-2), hydroxyapatite-tricalcium phosphate, hydroxyapatite-tricalcium phosphate plus rhBMP-2, and no scaffold. New bone formation was assessed radiomorphometrically and histomorphometrically at 4, 8, and 12 weeks. RESULTS: Radiomorphometrically, untreated animals formed 43 +/- 6 percent, 53 +/- 8 percent, and 48 +/- 3 percent new bone at 4, 8, and 12 weeks, respectively. Animals treated with absorbable collagen sponge formed 50 +/- 6 percent, 79 +/- 9 percent, and 69 +/- 7 percent new bone, respectively. Absorbable collagen sponge plus rhBMP-2-treated animals formed 49 +/- 2 percent, 71 +/- 6 percent, and 66 +/- 7 percent new bone, respectively. Hydroxyapatite-tricalcium phosphate treatment stimulated 69 +/- 12 percent, 86 +/- 3 percent (p < 0.05), and 87 +/- 14 percent new bone, respectively. Histomorphometry demonstrated an increase in bone formation in animals treated with hydroxyapatite-tricalcium phosphate plus rhBMP-2 (p < 0.05; 4 weeks) compared with empty scaffold. CONCLUSIONS: Radiomorphometrically, absorbable collagen sponge and hydroxyapatite-tricalcium phosphate scaffolds induced more bone formation than untreated controls. The rhBMP-2 added a small but significant histomorphometric osteogenic advantage to the hydroxyapatite-tricalcium phosphate scaffold.

Duke Scholars

Published In

Plast Reconstr Surg

DOI

EISSN

1529-4242

Publication Date

December 2009

Volume

124

Issue

6

Start / End Page

1829 / 1839

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta
  • Surgery
  • Risk Factors
  • Recombinant Proteins
  • Rats, Sprague-Dawley
  • Rats
  • Random Allocation
  • Plastic Surgery Procedures
  • Periosteum
  • Osteogenesis
 

Citation

APA
Chicago
ICMJE
MLA
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Nguyen, P. D., Lin, C. D., Allori, A. C., Schachar, J. S., Ricci, J. L., Saadeh, P. B., & Warren, S. M. (2009). Scaffold-based rhBMP-2 therapy in a rat alveolar defect model: implications for human gingivoperiosteoplasty. Plast Reconstr Surg, 124(6), 1829–1839. https://doi.org/10.1097/PRS.0b013e3181bf8024
Nguyen, Phuong D., Clarence D. Lin, Alexander C. Allori, Jeffrey S. Schachar, John L. Ricci, Pierre B. Saadeh, and Stephen M. Warren. “Scaffold-based rhBMP-2 therapy in a rat alveolar defect model: implications for human gingivoperiosteoplasty.Plast Reconstr Surg 124, no. 6 (December 2009): 1829–39. https://doi.org/10.1097/PRS.0b013e3181bf8024.
Nguyen PD, Lin CD, Allori AC, Schachar JS, Ricci JL, Saadeh PB, et al. Scaffold-based rhBMP-2 therapy in a rat alveolar defect model: implications for human gingivoperiosteoplasty. Plast Reconstr Surg. 2009 Dec;124(6):1829–39.
Nguyen, Phuong D., et al. “Scaffold-based rhBMP-2 therapy in a rat alveolar defect model: implications for human gingivoperiosteoplasty.Plast Reconstr Surg, vol. 124, no. 6, Dec. 2009, pp. 1829–39. Pubmed, doi:10.1097/PRS.0b013e3181bf8024.
Nguyen PD, Lin CD, Allori AC, Schachar JS, Ricci JL, Saadeh PB, Warren SM. Scaffold-based rhBMP-2 therapy in a rat alveolar defect model: implications for human gingivoperiosteoplasty. Plast Reconstr Surg. 2009 Dec;124(6):1829–1839.

Published In

Plast Reconstr Surg

DOI

EISSN

1529-4242

Publication Date

December 2009

Volume

124

Issue

6

Start / End Page

1829 / 1839

Location

United States

Related Subject Headings

  • Transforming Growth Factor beta
  • Surgery
  • Risk Factors
  • Recombinant Proteins
  • Rats, Sprague-Dawley
  • Rats
  • Random Allocation
  • Plastic Surgery Procedures
  • Periosteum
  • Osteogenesis