Methods to generate genetically engineered mouse models of soft tissue sarcoma.

Journal Article (Journal Article)

We discuss the generation of primary soft tissue sarcomas in mice using the Cre-loxP system to activate conditional mutations in oncogenic Kras and the tumor suppressor p53 (LSL-Kras(G12D/+); p53(flox/flox)). Sarcomas can be generated either by adenoviral delivery of Cre recombinase, activation of transgenic Cre recombinase with tamoxifen, or through transplantation of isolated satellite cells with Cre activation in vitro. Various applications of these models are discussed, including anticancer therapies, metastasis, in vivo imaging, and genetic requirements for tumorigenesis.

Full Text

Duke Authors

Cited Authors

  • Dodd, RD; Añó, L; Blum, JM; Li, Z; Van Mater, D; Kirsch, DG

Published Date

  • 2015

Published In

Volume / Issue

  • 1267 /

Start / End Page

  • 283 - 295

PubMed ID

  • 25636474

Pubmed Central ID

  • PMC5612825

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-2297-0_13


  • eng

Conference Location

  • United States