Developmental regulation of apical endocytosis controls epithelial patterning in vertebrate tubular organs.

Journal Article

Epithelial organs develop through tightly coordinated events of cell proliferation and differentiation in which endocytosis plays a major role. Despite recent advances, how endocytosis regulates the development of vertebrate organs is still unknown. Here we describe a mechanism that facilitates the apical availability of endosomal SNARE receptors for epithelial morphogenesis through the developmental upregulation of plasmolipin (pllp) in a highly endocytic segment of the zebrafish posterior midgut. The protein PLLP (Pllp in fish) recruits the clathrin adaptor EpsinR to sort the SNARE machinery of the endolysosomal pathway into the subapical compartment, which is a switch for polarized endocytosis. Furthermore, PLLP expression induces apical Crumbs internalization and the activation of the Notch signalling pathway, both crucial steps in the acquisition of cell polarity and differentiation of epithelial cells. We thus postulate that differential apical endosomal SNARE sorting is a mechanism that regulates epithelial patterning.

Full Text

Duke Authors

Cited Authors

  • Rodríguez-Fraticelli, AE; Bagwell, J; Bosch-Fortea, M; Boncompain, G; Reglero-Real, N; García-León, MJ; Andrés, G; Toribio, ML; Alonso, MA; Millán, J; Perez, F; Bagnat, M; Martín-Belmonte, F

Published Date

  • March 2015

Published In

Volume / Issue

  • 17 / 3

Start / End Page

  • 241 - 250

PubMed ID

  • 25706235

Electronic International Standard Serial Number (EISSN)

  • 1476-4679

International Standard Serial Number (ISSN)

  • 1465-7392

Digital Object Identifier (DOI)

  • 10.1038/ncb3106

Language

  • eng