Pulse Field Gel Electrophoresis.

Published

Journal Article

Pulse Field Gel Electrophoresis (PFGE) is a powerful genotyping technique used for the separation of large DNA molecules (entire genomic DNA) after digesting it with unique restriction enzymes and applying to a gel matrix under the electric field that periodically changes direction. PFGE is a variation of agarose gel electrophoresis that permits analysis of bacterial DNA fragments over an order of magnitude larger than that with conventional restriction enzyme analysis. It provides a good representation of the entire bacterial chromosome in a single gel with a highly reproducible restriction profile, providing clearly distinct and well-resolved DNA fragments.

Full Text

Duke Authors

Cited Authors

  • Sharma-Kuinkel, BK; Rude, TH; Fowler, VG

Published Date

  • 2016

Published In

Volume / Issue

  • 1373 /

Start / End Page

  • 117 - 130

PubMed ID

  • 25682374

Pubmed Central ID

  • 25682374

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/7651_2014_191

Language

  • eng

Conference Location

  • United States