Pulse Field Gel Electrophoresis.
Journal Article (Journal Article)
Pulse Field Gel Electrophoresis (PFGE) is a powerful genotyping technique used for the separation of large DNA molecules (entire genomic DNA) after digesting it with unique restriction enzymes and applying to a gel matrix under the electric field that periodically changes direction. PFGE is a variation of agarose gel electrophoresis that permits analysis of bacterial DNA fragments over an order of magnitude larger than that with conventional restriction enzyme analysis. It provides a good representation of the entire bacterial chromosome in a single gel with a highly reproducible restriction profile, providing clearly distinct and well-resolved DNA fragments.
Full Text
Duke Authors
Cited Authors
- Sharma-Kuinkel, BK; Rude, TH; Fowler, VG
Published Date
- 2016
Published In
Volume / Issue
- 1373 /
Start / End Page
- 117 - 130
PubMed ID
- 25682374
Pubmed Central ID
- 25682374
Electronic International Standard Serial Number (EISSN)
- 1940-6029
Digital Object Identifier (DOI)
- 10.1007/7651_2014_191
Language
- eng
Conference Location
- United States