A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.

Journal Article

Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR-Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.

Full Text

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Duke Authors

Gersbach, Charles

Cited Authors

Polstein, LR; Gersbach, CA

Published Date

March 2015

Published In

Nature Chemical Biology

Volume / Issue

11 / 3

Start / End Page

198 - 200

PubMed ID

25664691

Electronic International Standard Serial Number (EISSN)

1552-4469

International Standard Serial Number (ISSN)

1552-4450

Digital Object Identifier (DOI)

10.1038/nchembio.1753

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