A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.

Journal Article

Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR-Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.

Full Text

Duke Authors

Cited Authors

  • Polstein, LR; Gersbach, CA

Published Date

  • March 2015

Published In

Volume / Issue

  • 11 / 3

Start / End Page

  • 198 - 200

PubMed ID

  • 25664691

Electronic International Standard Serial Number (EISSN)

  • 1552-4469

International Standard Serial Number (ISSN)

  • 1552-4450

Digital Object Identifier (DOI)

  • 10.1038/nchembio.1753

Language

  • eng