A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.
Journal Article (Journal Article)
Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR-Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.
Full Text
Duke Authors
Cited Authors
- Polstein, LR; Gersbach, CA
Published Date
- March 2015
Published In
Volume / Issue
- 11 / 3
Start / End Page
- 198 - 200
PubMed ID
- 25664691
Pubmed Central ID
- PMC4412021
Electronic International Standard Serial Number (EISSN)
- 1552-4469
International Standard Serial Number (ISSN)
- 1552-4450
Digital Object Identifier (DOI)
- 10.1038/nchembio.1753
Language
- eng