Reliable genotypic tropism tests for the major HIV-1 subtypes.

Journal Article

Over the past decade antiretroviral drugs have dramatically improved the prognosis for HIV-1 infected individuals, yet achieving better access to vulnerable populations remains a challenge. The principal obstacle to the CCR5-antagonist, maraviroc, from being more widely used in anti-HIV-1 therapy regimens is that the pre-treatment genotypic "tropism tests" to determine virus susceptibility to maraviroc have been developed primarily for HIV-1 subtype B strains, which account for only 10% of infections worldwide. We therefore developed PhenoSeq, a suite of HIV-1 genotypic tropism assays that are highly sensitive and specific for establishing the tropism of HIV-1 subtypes A, B, C, D and circulating recombinant forms of subtypes AE and AG, which together account for 95% of HIV-1 infections worldwide. The PhenoSeq platform will inform the appropriate use of maraviroc and future CCR5 blocking drugs in regions of the world where non-B HIV-1 predominates, which are burdened the most by the HIV-1 pandemic.

Full Text

Duke Authors

Cited Authors

  • Cashin, K; Gray, LR; Harvey, KL; Perez-Bercoff, D; Lee, GQ; Sterjovski, J; Roche, M; Demarest, JF; Drummond, F; Harrigan, PR; Churchill, MJ; Gorry, PR

Published Date

  • February 25, 2015

Published In

Volume / Issue

  • 5 /

Start / End Page

  • 8543 -

PubMed ID

  • 25712827

Electronic International Standard Serial Number (EISSN)

  • 2045-2322

International Standard Serial Number (ISSN)

  • 2045-2322

Digital Object Identifier (DOI)

  • 10.1038/srep08543

Language

  • eng