Use of steady-state fluorescence anisotropy with PEBBLE nanosensors for chemical analysis
Published
Conference Paper
In this study we show that steady-state fluorescence anisotropy within PEBBLEs (Probes Encapsulated By Biologically Localized Embedding) can be used for the optochemical sensing of analytes such as Zn2+, O2, and Ca2+. Steady-state fluorescence anisotropy is a non-time resolved method that measures a combination of rotational and fluorescence lifetimes. This eliminates the need for reference dyes and ratiometric techniques to obtain quantitative results, even when using intensity-based sensor dyes. An advantage to working with PEBBLE nanosensors is that the encapsulated dye is localized in a constant rotational environment. This is in contrast to the use of free dyes, which can be affected by interferents such as protein binding.
Full Text
Duke Authors
Cited Authors
- Horvath, T; Monson, E; Sumner, J; Xu, H; Kopelman, R
Published Date
- January 1, 2002
Published In
Volume / Issue
- 4626 /
Start / End Page
- 486 - 492
International Standard Serial Number (ISSN)
- 0277-786X
Digital Object Identifier (DOI)
- 10.1117/12.472115
Citation Source
- Scopus