Use of steady-state fluorescence anisotropy with PEBBLE nanosensors for chemical analysis

Conference Paper

In this study we show that steady-state fluorescence anisotropy within PEBBLEs (Probes Encapsulated By Biologically Localized Embedding) can be used for the optochemical sensing of analytes such as Zn2+, O2, and Ca2+. Steady-state fluorescence anisotropy is a non-time resolved method that measures a combination of rotational and fluorescence lifetimes. This eliminates the need for reference dyes and ratiometric techniques to obtain quantitative results, even when using intensity-based sensor dyes. An advantage to working with PEBBLE nanosensors is that the encapsulated dye is localized in a constant rotational environment. This is in contrast to the use of free dyes, which can be affected by interferents such as protein binding.

Full Text

Duke Authors

Cited Authors

  • Horvath, T; Monson, E; Sumner, J; Xu, H; Kopelman, R

Published Date

  • January 1, 2002

Published In

Volume / Issue

  • 4626 /

Start / End Page

  • 486 - 492

International Standard Serial Number (ISSN)

  • 0277-786X

Digital Object Identifier (DOI)

  • 10.1117/12.472115

Citation Source

  • Scopus