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Flexible digital signal processing architecture for narrowband and spread-spectrum lock-in detection in multiphoton microscopy and time-resolved spectroscopy

Publication ,  Journal Article
Wilson, JW; Park, JK; Warren, WS; Fischer, MC
Published in: Review of Scientific Instruments
March 1, 2015

The lock-in amplifier is a critical component in many different types of experiments, because of its ability to reduce spurious or environmental noise components by restricting detection to a single frequency and phase. One example application is pump-probe microscopy, a multiphoton technique that leverages excited-state dynamics for imaging contrast. With this application in mind, we present here the design and implementation of a high-speed lock-in amplifier on the field-programmable gate array (FPGA) coprocessor of a data acquisition board. The most important advantage is the inherent ability to filter signals based on more complex modulation patterns. As an example, we use the flexibility of the FPGA approach to enable a novel pump-probe detection scheme based on spread-spectrum communications techniques.

Duke Scholars

Published In

Review of Scientific Instruments

DOI

EISSN

1089-7623

ISSN

0034-6748

Publication Date

March 1, 2015

Volume

86

Issue

3

Publisher

AIP Publishing

Related Subject Headings

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  • Signal-To-Noise Ratio
  • Signal Processing, Computer-Assisted
  • Rhodamines
  • Porphyrins
  • Optical Imaging
  • Microscopy
  • Mice, Inbred C57BL
  • Melanins
 

Citation

APA
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MLA
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Wilson, J. W., Park, J. K., Warren, W. S., & Fischer, M. C. (2015). Flexible digital signal processing architecture for narrowband and spread-spectrum lock-in detection in multiphoton microscopy and time-resolved spectroscopy. Review of Scientific Instruments, 86(3). https://doi.org/10.1063/1.4916261
Wilson, Jesse W., Jong Kang Park, Warren S. Warren, and Martin C. Fischer. “Flexible digital signal processing architecture for narrowband and spread-spectrum lock-in detection in multiphoton microscopy and time-resolved spectroscopy.” Review of Scientific Instruments 86, no. 3 (March 1, 2015). https://doi.org/10.1063/1.4916261.
Wilson, Jesse W., et al. “Flexible digital signal processing architecture for narrowband and spread-spectrum lock-in detection in multiphoton microscopy and time-resolved spectroscopy.” Review of Scientific Instruments, vol. 86, no. 3, AIP Publishing, Mar. 2015. Crossref, doi:10.1063/1.4916261.
Wilson JW, Park JK, Warren WS, Fischer MC. Flexible digital signal processing architecture for narrowband and spread-spectrum lock-in detection in multiphoton microscopy and time-resolved spectroscopy. Review of Scientific Instruments. AIP Publishing; 2015 Mar 1;86(3).

Published In

Review of Scientific Instruments

DOI

EISSN

1089-7623

ISSN

0034-6748

Publication Date

March 1, 2015

Volume

86

Issue

3

Publisher

AIP Publishing

Related Subject Headings

  • Spectrum Analysis
  • Skin
  • Signal-To-Noise Ratio
  • Signal Processing, Computer-Assisted
  • Rhodamines
  • Porphyrins
  • Optical Imaging
  • Microscopy
  • Mice, Inbred C57BL
  • Melanins