Skip to main content

Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung.

Publication ,  Journal Article
Jain, R; Barkauskas, CE; Takeda, N; Bowie, EJ; Aghajanian, H; Wang, Q; Padmanabhan, A; Manderfield, LJ; Gupta, M; Li, D; Li, L; Trivedi, CM ...
Published in: Nat Commun
April 13, 2015

The plasticity of differentiated cells in adult tissues undergoing repair is an area of intense research. Pulmonary alveolar type II cells produce surfactant and function as progenitors in the adult, demonstrating both self-renewal and differentiation into gas exchanging type I cells. In vivo, type I cells are thought to be terminally differentiated and their ability to give rise to alternate lineages has not been reported. Here we show that Hopx becomes restricted to type I cells during development. However, unexpectedly, lineage-labelled Hopx(+) cells both proliferate and generate type II cells during adult alveolar regrowth following partial pneumonectomy. In clonal 3D culture, single Hopx(+) type I cells generate organoids composed of type I and type II cells, a process modulated by TGFβ signalling. These findings demonstrate unanticipated plasticity of type I cells and a bidirectional lineage relationship between distinct differentiated alveolar epithelial cell types in vivo and in single-cell culture.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

April 13, 2015

Volume

6

Start / End Page

6727

Location

England

Related Subject Headings

  • Transforming Growth Factor beta
  • Tamoxifen
  • Signal Transduction
  • Regeneration
  • Pulmonary Alveoli
  • Pneumonectomy
  • Mice, Transgenic
  • Mice
  • Male
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Jain, R., Barkauskas, C. E., Takeda, N., Bowie, E. J., Aghajanian, H., Wang, Q., … Epstein, J. A. (2015). Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung. Nat Commun, 6, 6727. https://doi.org/10.1038/ncomms7727
Jain, Rajan, Christina E. Barkauskas, Norifumi Takeda, Emily J. Bowie, Haig Aghajanian, Qiaohong Wang, Arun Padmanabhan, et al. “Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung.Nat Commun 6 (April 13, 2015): 6727. https://doi.org/10.1038/ncomms7727.
Jain R, Barkauskas CE, Takeda N, Bowie EJ, Aghajanian H, Wang Q, et al. Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung. Nat Commun. 2015 Apr 13;6:6727.
Jain, Rajan, et al. “Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung.Nat Commun, vol. 6, Apr. 2015, p. 6727. Pubmed, doi:10.1038/ncomms7727.
Jain R, Barkauskas CE, Takeda N, Bowie EJ, Aghajanian H, Wang Q, Padmanabhan A, Manderfield LJ, Gupta M, Li D, Li L, Trivedi CM, Hogan BLM, Epstein JA. Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung. Nat Commun. 2015 Apr 13;6:6727.

Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

April 13, 2015

Volume

6

Start / End Page

6727

Location

England

Related Subject Headings

  • Transforming Growth Factor beta
  • Tamoxifen
  • Signal Transduction
  • Regeneration
  • Pulmonary Alveoli
  • Pneumonectomy
  • Mice, Transgenic
  • Mice
  • Male
  • Humans