Macrophage Phagocytosis Assay of Staphylococcus aureus by Flow Cytometry.

Journal Article (Journal Article)

This protocol describes a straightforward technique to evaluate the phagocytotic capacity of murine macrophages for Staphylococcus aureus (S. aureus). By staining S. aureus with Hexidium Iodide and staining murine bone marrow-derived macrophages (BMDMs) with FITC, the macrophage bacterial up-taking ability can be rapidly analyzed by flow cytometry. S. aureus is a Gram-positive bacteria causing severe human and animal infections. Host immune cells such as macrophages serve to eliminate S. aureus by phagocytosing the pathogen and save the host from life-threatening diseases. Study of host macrophage ability to phagocytose S. aureus is important for understanding the host-pathogen interaction and can help to elucidate the pathogenesis of S. aureus infection. This protocol may also be applied for macrophage phagocytotic assay of other gram-positive bacteria.

Full Text

Duke Authors

Cited Authors

  • Yan, Q; Ahn, SH; Fowler, VG

Published Date

  • February 20, 2015

Published In

Volume / Issue

  • 5 / 4

PubMed ID

  • 25879046

Pubmed Central ID

  • PMC4394738

International Standard Serial Number (ISSN)

  • 2331-8325

Digital Object Identifier (DOI)

  • 10.21769/bioprotoc.1406


  • eng

Conference Location

  • United States