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Single-Step Assembly of Multimodal Imaging Nanocarriers: MRI and Long-Wavelength Fluorescence Imaging.

Publication ,  Journal Article
Pinkerton, NM; Gindy, ME; Calero-DdelC, VL; Wolfson, T; Pagels, RF; Adler, D; Gao, D; Li, S; Wang, R; Zevon, M; Yao, N; Pacheco, C; Sinko, PJ ...
Published in: Advanced healthcare materials
June 2015

Magnetic resonance imaging (MRI)- and near-infrared (NIR)-active, multimodal composite nanocarriers (CNCs) are prepared using a simple one-step process, flash nanoprecipitation (FNP). The FNP process allows for the independent control of the hydrodynamic diameter, co-core excipient and NIR dye loading, and iron oxide-based nanocrystal (IONC) content of the CNCs. In the controlled precipitation process, 10 nm IONCs are encapsulated into poly(ethylene glycol) (PEG) stabilized CNCs to make biocompatible T2 contrast agents. By adjusting the formulation, CNC size is tuned between 80 and 360 nm. Holding the CNC size constant at an intensity weighted average diameter of 99 ± 3 nm (PDI width 28 nm), the particle relaxivity varies linearly with encapsulated IONC content ranging from 66 to 533 × 10(-3) m(-1) s(-1) for CNCs formulated with 4-16 wt% IONC. To demonstrate the use of CNCs as in vivo MRI contrast agents, CNCs are surface functionalized with liver-targeting hydroxyl groups. The CNCs enable the detection of 0.8 mm(3) non-small cell lung cancer metastases in mice livers via MRI. Incorporating the hydrophobic, NIR dye tris-(porphyrinato)zinc(II) into CNCs enables complementary visualization with long-wavelength fluorescence at 800 nm. In vivo imaging demonstrates the ability of CNCs to act both as MRI and fluorescent imaging agents.

Duke Scholars

Published In

Advanced healthcare materials

DOI

EISSN

2192-2659

ISSN

2192-2640

Publication Date

June 2015

Volume

4

Issue

9

Start / End Page

1376 / 1385

Related Subject Headings

  • Polyethylene Glycols
  • Particle Size
  • Optical Imaging
  • Nanoparticles
  • Mice
  • Magnetic Resonance Imaging
  • Fluorescent Dyes
  • Ferric Compounds
  • Drug Carriers
  • Contrast Media
 

Citation

APA
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Pinkerton, N. M., Gindy, M. E., Calero-DdelC, V. L., Wolfson, T., Pagels, R. F., Adler, D., … Prud’homme, R. K. (2015). Single-Step Assembly of Multimodal Imaging Nanocarriers: MRI and Long-Wavelength Fluorescence Imaging. Advanced Healthcare Materials, 4(9), 1376–1385. https://doi.org/10.1002/adhm.201400766
Pinkerton, Nathalie M., Marian E. Gindy, Victoria L. Calero-DdelC, Theodore Wolfson, Robert F. Pagels, Derek Adler, Dayuan Gao, et al. “Single-Step Assembly of Multimodal Imaging Nanocarriers: MRI and Long-Wavelength Fluorescence Imaging.Advanced Healthcare Materials 4, no. 9 (June 2015): 1376–85. https://doi.org/10.1002/adhm.201400766.
Pinkerton NM, Gindy ME, Calero-DdelC VL, Wolfson T, Pagels RF, Adler D, et al. Single-Step Assembly of Multimodal Imaging Nanocarriers: MRI and Long-Wavelength Fluorescence Imaging. Advanced healthcare materials. 2015 Jun;4(9):1376–85.
Pinkerton, Nathalie M., et al. “Single-Step Assembly of Multimodal Imaging Nanocarriers: MRI and Long-Wavelength Fluorescence Imaging.Advanced Healthcare Materials, vol. 4, no. 9, June 2015, pp. 1376–85. Epmc, doi:10.1002/adhm.201400766.
Pinkerton NM, Gindy ME, Calero-DdelC VL, Wolfson T, Pagels RF, Adler D, Gao D, Li S, Wang R, Zevon M, Yao N, Pacheco C, Therien MJ, Rinaldi C, Sinko PJ, Prud’homme RK. Single-Step Assembly of Multimodal Imaging Nanocarriers: MRI and Long-Wavelength Fluorescence Imaging. Advanced healthcare materials. 2015 Jun;4(9):1376–1385.
Journal cover image

Published In

Advanced healthcare materials

DOI

EISSN

2192-2659

ISSN

2192-2640

Publication Date

June 2015

Volume

4

Issue

9

Start / End Page

1376 / 1385

Related Subject Headings

  • Polyethylene Glycols
  • Particle Size
  • Optical Imaging
  • Nanoparticles
  • Mice
  • Magnetic Resonance Imaging
  • Fluorescent Dyes
  • Ferric Compounds
  • Drug Carriers
  • Contrast Media