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Abstract 3536: Roles of miR-215 and regulatory mechanisms for its biogenesis in response to hypoxia in glioblastoma stem cells

Publication ,  Conference
Hu, J; Sun, T; Wang, H; Wang, P; Fu, X-D; Li, Q-J; Wang, X-F
Published in: Cancer Research
October 1, 2014

Glioblastoma (GBM) is the most common and aggressive primary brain tumor in adults, which is highly resistant to conventional therapies. In GBM, glioblastoma stem cells (GSCs) are critical for the tumor progression and resistance. The hypoxic tumor microenvironment has been reported to help maintain GSCs. However, the mechanism regulating responses of GSCs to hypoxia remains elusive. Abnormal microRNA processing is a contributing factor in various types of cancer including GBM. Using small RNA deep sequencing and Real-time PCR array, we found a group of miRNAs altered in the expression levels under hypoxia in the CD133+ GSCs. Further through a functional screen, we identified miR-215 as an important mediator for GSC growth and tumor progression in response to hypoxia. In addition, we also investigated the regulatory mechanisms for the biogenesis of the altered miRNAs under hypoxia. Interestingly, we found that hypoxia could regulate miRNA processing both transcriptionally and post-transcriptionally. These findings suggest that de-regulated miRNA biogenesis may have significant impacts on the activities of GSCs under hypoxia.Citation Format: Jing Hu, Tao Sun, Hui Wang, Pingping Wang, Xiang-Dong Fu, Qi-Jing Li, Xiao-Fan Wang. Roles of miR-215 and regulatory mechanisms for its biogenesis in response to hypoxia in glioblastoma stem cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3536. doi:10.1158/1538-7445.AM2014-3536

Duke Scholars

Published In

Cancer Research

DOI

EISSN

1538-7445

ISSN

0008-5472

Publication Date

October 1, 2014

Volume

74

Issue

19_Supplement

Start / End Page

3536 / 3536

Publisher

American Association for Cancer Research (AACR)

Related Subject Headings

  • Oncology & Carcinogenesis
  • 3211 Oncology and carcinogenesis
  • 3101 Biochemistry and cell biology
  • 1112 Oncology and Carcinogenesis
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hu, J., Sun, T., Wang, H., Wang, P., Fu, X.-D., Li, Q.-J., & Wang, X.-F. (2014). Abstract 3536: Roles of miR-215 and regulatory mechanisms for its biogenesis in response to hypoxia in glioblastoma stem cells. In Cancer Research (Vol. 74, pp. 3536–3536). American Association for Cancer Research (AACR). https://doi.org/10.1158/1538-7445.am2014-3536
Hu, Jing, Tao Sun, Hui Wang, Pingping Wang, Xiang-Dong Fu, Qi-Jing Li, and Xiao-Fan Wang. “Abstract 3536: Roles of miR-215 and regulatory mechanisms for its biogenesis in response to hypoxia in glioblastoma stem cells.” In Cancer Research, 74:3536–3536. American Association for Cancer Research (AACR), 2014. https://doi.org/10.1158/1538-7445.am2014-3536.
Hu J, Sun T, Wang H, Wang P, Fu X-D, Li Q-J, et al. Abstract 3536: Roles of miR-215 and regulatory mechanisms for its biogenesis in response to hypoxia in glioblastoma stem cells. In: Cancer Research. American Association for Cancer Research (AACR); 2014. p. 3536–3536.
Hu, Jing, et al. “Abstract 3536: Roles of miR-215 and regulatory mechanisms for its biogenesis in response to hypoxia in glioblastoma stem cells.” Cancer Research, vol. 74, no. 19_Supplement, American Association for Cancer Research (AACR), 2014, pp. 3536–3536. Crossref, doi:10.1158/1538-7445.am2014-3536.
Hu J, Sun T, Wang H, Wang P, Fu X-D, Li Q-J, Wang X-F. Abstract 3536: Roles of miR-215 and regulatory mechanisms for its biogenesis in response to hypoxia in glioblastoma stem cells. Cancer Research. American Association for Cancer Research (AACR); 2014. p. 3536–3536.

Published In

Cancer Research

DOI

EISSN

1538-7445

ISSN

0008-5472

Publication Date

October 1, 2014

Volume

74

Issue

19_Supplement

Start / End Page

3536 / 3536

Publisher

American Association for Cancer Research (AACR)

Related Subject Headings

  • Oncology & Carcinogenesis
  • 3211 Oncology and carcinogenesis
  • 3101 Biochemistry and cell biology
  • 1112 Oncology and Carcinogenesis