In vivo pump-probe microscopy of melanoma: Characterizing shifts in excited state photodynamics with respect to invasiveness

Conference Paper

© 2015 SPIE. Pump-probe microscopy is a multiphoton technique that generates molecular contrast from absorptive pigments, such as melanin. It holds the potential to be used as a non-invasive screening tool to discern whether a given early-stage melanoma has acquired the capacity for metastasis. Here, we examined lesions in a Braf(V600E)-driven model of melanoma to assess whether loss of the tumor suppressor gene Pten in a is accompanied by a shift in pigment expression, as measured in vivo by pump-probe microscopy. The data were analyzed to determine differences in the excited-state lifetime of melanins expressed in Pten-competent and Pten-loss pigmented lesions. Loss of the tumor suppressor Pten was found to be accompanied by a statistically significant decrease in pixel-average excited state lifetime (p = 1.3e-4).

Full Text

Duke Authors

Cited Authors

  • Wilson, JW; Degan, S; Gainey, CS; Deb, S; Dall, CP; Tameze-Rivas, Y; Zhang, J; Warren, WS

Published Date

  • January 1, 2015

Published In

Volume / Issue

  • 9329 /

International Standard Serial Number (ISSN)

  • 1605-7422

International Standard Book Number 13 (ISBN-13)

  • 9781628414196

Digital Object Identifier (DOI)

  • 10.1117/12.2079886

Citation Source

  • Scopus