In vivo pump-probe microscopy of melanoma: Characterizing shifts in excited state photodynamics with respect to invasiveness
Published
Conference Paper
© 2015 SPIE. Pump-probe microscopy is a multiphoton technique that generates molecular contrast from absorptive pigments, such as melanin. It holds the potential to be used as a non-invasive screening tool to discern whether a given early-stage melanoma has acquired the capacity for metastasis. Here, we examined lesions in a Braf(V600E)-driven model of melanoma to assess whether loss of the tumor suppressor gene Pten in a is accompanied by a shift in pigment expression, as measured in vivo by pump-probe microscopy. The data were analyzed to determine differences in the excited-state lifetime of melanins expressed in Pten-competent and Pten-loss pigmented lesions. Loss of the tumor suppressor Pten was found to be accompanied by a statistically significant decrease in pixel-average excited state lifetime (p = 1.3e-4).
Full Text
Duke Authors
Cited Authors
- Wilson, JW; Degan, S; Gainey, CS; Deb, S; Dall, CP; Tameze-Rivas, Y; Zhang, J; Warren, WS
Published Date
- January 1, 2015
Published In
Volume / Issue
- 9329 /
International Standard Serial Number (ISSN)
- 1605-7422
International Standard Book Number 13 (ISBN-13)
- 9781628414196
Digital Object Identifier (DOI)
- 10.1117/12.2079886
Citation Source
- Scopus