Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots.
BACKGROUND:Trimethoprim-sulfamethoxazole (TMP-SMX) is an antimicrobial drug combination commonly prescribed in children and adults. The study objectives were to validate and apply an HPLC-MS/MS method to quantify TMP-SMX in dried plasma spots (DPS) and dried urine spots (DUS), and perform a comparability analysis with liquid matrices. RESULTS:For TMP the validated range was 100-50,000 ng/ml for DPS and 500-250,000 ng/ml for DUS; for SMX, the validated range was 1000-500,000 ng/ml for both DPS and DUS. Good agreement was noted between DPS/DUS and liquid plasma and urine samples for TMP, while only modest agreement was observed for SMX in both matrices. CONCLUSION:A precise, accurate and reproducible method was developed to quantify TMP-SMX in DPS and DUS samples.
Gonzalez, D; Melloni, C; Poindexter, BB; Yogev, R; Atz, AM; Sullivan, JE; Mendley, SR; Delmore, P; Delinsky, A; Zimmerman, K; Lewandowski, A; Harper, B; Lewis, KC; Benjamin, DK; Cohen-Wolkowiez, M; Best Pharmaceuticals for Children Act--Pediatric Trials Network Administrative Core Committee,
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