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Abstract 518: Identification of differentially expressed microRNAs in human hepatocellular adenoma associated with type I glycogen storage disease: a potential utility as biomarkers

Publication ,  Conference
Li, L-H; Chiu, L-Y; Kishnani, PS; Chuang, T-P; Tang, C-Y; Liu, C-Y; Bali, D; Koeberl, D; Austin, S; Boyette, K; Weinstein, DA; Murphy, E ...
Published in: Cancer Research
October 1, 2014

Background: It is known that malignant transformation to hepatocellular carcinoma (HCC) occurs at a higher frequency in hepatocellular adenoma (HCA) from type I glycogen storage disease (GSD I) compared to HCA from other etiologies. In this study, we aimed to identify differentially expressed miRNAs in GSD Ia HCA as candidates that could serve as putative biomarkers for detection of GSD Ia HCA and/or risk assessment of malignant transformation.Methods: Utilizing massively parallel sequencing, the miRNA profiling was performed for paired adenomas and normal liver tissues from seven GSD Ia patients. Differentially expressed miRNAs were validated in liver tumor tissues, HCC cell lines and serum using quantitative RT-PCR.Results: miR-34a, miR-34a*, miR-224, miR-224*, miR-424, miR-452 and miR-455-5p were found to be commonly deregulated in GSD Ia HCA, general population HCA, and HCC cell lines at compatible levels. In comparison with GSD Ia HCA, the upregulation of miR-130b and downregulation of miR-199a-5p, miR-199b-5p, and miR-214 were more significant in HCC cell lines. Furthermore, serum level of miR-130b in GSD Ia patients with HCA was moderately higher than that in either GSD Ia patients without HCA or healthy individuals.Conclusion: We make the first observation of the distinct miRNA deregulation in HCA associated with GSD Ia. We also provide evidence that miR-130b could serve as a circulating biomarker for detection of GSD Ia HCA. This work provides prominent candidate miRNAs worth evaluating as biomarkers for monitoring the development and progress of liver tumors in GSD Ia patients in the future.Citation Format: Ling-Hui Li, Li-Ya Chiu, Priya S. Kishnani, Tzu-Po Chuang, Cheng-Yang Tang, Cheng-Yuan Liu, Deeksha Bali, Dwight Koeberl, Stephanie Austin, Keri Boyette, David A. Weinstein, Elaine Murphy, Adam Yao, Yuan-Tsong Chen. Identification of differentially expressed microRNAs in human hepatocellular adenoma associated with type I glycogen storage disease: a potential utility as biomarkers. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 518. doi:10.1158/1538-7445.AM2014-518

Duke Scholars

Published In

Cancer Research

DOI

EISSN

1538-7445

ISSN

0008-5472

Publication Date

October 1, 2014

Volume

74

Issue

19_Supplement

Start / End Page

518 / 518

Publisher

American Association for Cancer Research (AACR)

Related Subject Headings

  • Oncology & Carcinogenesis
  • 3211 Oncology and carcinogenesis
  • 3101 Biochemistry and cell biology
  • 1112 Oncology and Carcinogenesis
 

Citation

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Li, L.-H., Chiu, L.-Y., Kishnani, P. S., Chuang, T.-P., Tang, C.-Y., Liu, C.-Y., … Chen, Y.-T. (2014). Abstract 518: Identification of differentially expressed microRNAs in human hepatocellular adenoma associated with type I glycogen storage disease: a potential utility as biomarkers. In Cancer Research (Vol. 74, pp. 518–518). American Association for Cancer Research (AACR). https://doi.org/10.1158/1538-7445.am2014-518
Li, Ling-Hui, Li-Ya Chiu, Priya S. Kishnani, Tzu-Po Chuang, Cheng-Yang Tang, Cheng-Yuan Liu, Deeksha Bali, et al. “Abstract 518: Identification of differentially expressed microRNAs in human hepatocellular adenoma associated with type I glycogen storage disease: a potential utility as biomarkers.” In Cancer Research, 74:518–518. American Association for Cancer Research (AACR), 2014. https://doi.org/10.1158/1538-7445.am2014-518.
Li L-H, Chiu L-Y, Kishnani PS, Chuang T-P, Tang C-Y, Liu C-Y, et al. Abstract 518: Identification of differentially expressed microRNAs in human hepatocellular adenoma associated with type I glycogen storage disease: a potential utility as biomarkers. In: Cancer Research. American Association for Cancer Research (AACR); 2014. p. 518–518.
Li, Ling-Hui, et al. “Abstract 518: Identification of differentially expressed microRNAs in human hepatocellular adenoma associated with type I glycogen storage disease: a potential utility as biomarkers.” Cancer Research, vol. 74, no. 19_Supplement, American Association for Cancer Research (AACR), 2014, pp. 518–518. Crossref, doi:10.1158/1538-7445.am2014-518.
Li L-H, Chiu L-Y, Kishnani PS, Chuang T-P, Tang C-Y, Liu C-Y, Bali D, Koeberl D, Austin S, Boyette K, Weinstein DA, Murphy E, Yao A, Chen Y-T. Abstract 518: Identification of differentially expressed microRNAs in human hepatocellular adenoma associated with type I glycogen storage disease: a potential utility as biomarkers. Cancer Research. American Association for Cancer Research (AACR); 2014. p. 518–518.

Published In

Cancer Research

DOI

EISSN

1538-7445

ISSN

0008-5472

Publication Date

October 1, 2014

Volume

74

Issue

19_Supplement

Start / End Page

518 / 518

Publisher

American Association for Cancer Research (AACR)

Related Subject Headings

  • Oncology & Carcinogenesis
  • 3211 Oncology and carcinogenesis
  • 3101 Biochemistry and cell biology
  • 1112 Oncology and Carcinogenesis