A prochelator peptide designed to use heterometallic cooperativity to enhance metal ion affinity.

Published

Journal Article

A peptide has been designed so that its chelating affinity for one type of metal ion regulates its affinity for a second, different type of metal ion. The prochelator peptide (PCP), which is a fusion of motifs evocative of calcium loops and zinc fingers, forms a 1 : 2 Zn : peptide complex at pH 7.4 that increases its affinity for Zn2+ ∼3-fold in the presence of Tb3+ (log β2 from 13.8 to 14.3), while the 1 : 1 luminescent complex with Tb3+ is brighter, longer lived, and 20-fold tighter in the presence of Zn2+ (log K from 6.2 to 7.5). This unique example of cooperative, heterometallic allostery in a biologically compatible construct suggests the possibility of designing conditionally active metal-binding agents that could respond to dynamic changes in cellular metal status.

Full Text

Duke Authors

Cited Authors

  • Alies, B; Wiener, JD; Franz, KJ

Published Date

  • June 2015

Published In

Volume / Issue

  • 6 / 6

Start / End Page

  • 3606 - 3610

PubMed ID

  • 29511523

Pubmed Central ID

  • 29511523

Electronic International Standard Serial Number (EISSN)

  • 2041-6539

International Standard Serial Number (ISSN)

  • 2041-6520

Digital Object Identifier (DOI)

  • 10.1039/c5sc00602c

Language

  • eng