Deep tissue imaging using spectroscopic analysis of multiply scattered light

Journal Article (Journal Article)

Scattering limits the penetration depth of most optical imaging techniques. Efforts to overcome this limitation often require complex optical or computational schemes. We have developed a new method of assessing tissue properties based on spectroscopic analysis of multiply scattered light. The technique, multispectral multiple-scattering low-coherence interferometry (ms2/LCI), uses coherence and spatial gating to produce images of tissue optical properties up to 9 mm deep, with millimeter-scale resolution. The capabilities of ms2/LCI are demonstrated using tissue phantoms composed of chicken breast. Discrimination of diseased and healthy tissues is shown through imaging and analysis of burns in ex vivo human skin samples. Our technique may provide a powerful way to assess burn depth and progression in sensitive, burned tissues where physical contact is undesirable.

Full Text

Duke Authors

Cited Authors

  • Matthews, TE; Medina, M; Maher, JR; Levinson, H; Brown, WJ; Wax, A

Published Date

  • January 1, 2014

Published In

Volume / Issue

  • 1 / 2

Start / End Page

  • 105 - 111

International Standard Serial Number (ISSN)

  • 2334-2536

Digital Object Identifier (DOI)

  • 10.1364/OPTICA.1.000105

Citation Source

  • Scopus