Improved Angiogenesis in Response to Localized Delivery of Macrophage-Recruiting Molecules.

Journal Article (Journal Article)

Successful engineering of complex organs requires improved methods to promote rapid and stable vascularization of artificial tissue scaffolds. Toward this goal, tissue engineering strategies utilize the release of pro-angiogenic growth factors, alone or in combination, from biomaterials to induce angiogenesis. In this study we have used intravital microscopy to define key, dynamic cellular changes induced by the release of pro-angiogenic factors from polyethylene glycol diacrylate hydrogels transplanted in vivo. Our data show robust macrophage recruitment when the potent and synergistic angiogenic factors, PDGFBB and FGF2 were used as compared with VEGF alone and intravital imaging suggested roles for macrophages in endothelial tip cell migration and anastomosis, as well as pericyte-like behavior. Further data from in vivo experiments show that delivery of CSF1 with VEGF can dramatically improve the poor angiogenic response seen with VEGF alone. These studies show that incorporating macrophage-recruiting factors into the design of pro-angiogenic biomaterial scaffolds is a key strategy likely to be necessary for stable vascularization and survival of implanted artificial tissues.

Full Text

Duke Authors

Cited Authors

  • Hsu, C-W; Poché, RA; Saik, JE; Ali, S; Wang, S; Yosef, N; Calderon, GA; Scott, L; Vadakkan, TJ; Larina, IV; West, JL; Dickinson, ME

Published Date

  • January 2015

Published In

Volume / Issue

  • 10 / 7

Start / End Page

  • e0131643 -

PubMed ID

  • 26132702

Pubmed Central ID

  • PMC4489184

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

International Standard Serial Number (ISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0131643


  • eng