ADAM12: a genetic modifier of preclinical peripheral arterial disease.

Journal Article (Journal Article)

In prior studies from multiple groups, outcomes following experimental peripheral arterial disease (PAD) differed considerably across inbred mouse strains. Similarly, in humans with PAD, disease outcomes differ, even when there are similarities in risk factors, disease anatomy, arteriosclerotic burden, and hemodynamic measures. Previously, we identified a locus on mouse chromosome 7, limb salvage-associated quantitative trait locus 1 (LSq-1), which was sufficient to modify outcomes following experimental PAD. We compared expression of genes within LSq-1 in Balb/c mice, which normally show poor outcomes following experimental PAD, with that in C57Bl/6 mice, which normally show favorable outcomes, and found that a disintegrin and metalloproteinase gene 12 (ADAM12) had the most differential expression. Augmentation of ADAM12 expression in vivo improved outcomes following experimental PAD in Balb/c mice, whereas knockdown of ADAM12 made outcomes worse in C57Bl/6 mice. In vitro, ADAM12 expression modulates endothelial cell proliferation, survival, and angiogenesis in ischemia, and this appeared to be dependent on tyrosine kinase with Ig-like and EGF-like domain 2 (Tie2) activation. ADAM12 is sufficient to modify PAD severity in mice, and this likely occurs through regulation of Tie2.

Full Text

Duke Authors

Cited Authors

  • Dokun, AO; Chen, L; Okutsu, M; Farber, CR; Hazarika, S; Jones, WS; Craig, D; Marchuk, DA; Lye, RJ; Shah, SH; Annex, BH

Published Date

  • September 2015

Published In

Volume / Issue

  • 309 / 5

Start / End Page

  • H790 - H803

PubMed ID

  • 26163448

Pubmed Central ID

  • PMC4591412

Electronic International Standard Serial Number (EISSN)

  • 1522-1539

Digital Object Identifier (DOI)

  • 10.1152/ajpheart.00803.2014


  • eng

Conference Location

  • United States