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CNS myelin wrapping is driven by actin disassembly.

Publication ,  Journal Article
Zuchero, JB; Fu, M-M; Sloan, SA; Ibrahim, A; Olson, A; Zaremba, A; Dugas, JC; Wienbar, S; Caprariello, AV; Kantor, C; Leonoudakis, D; Gertz, K ...
Published in: Dev Cell
July 27, 2015

Myelin is essential in vertebrates for the rapid propagation of action potentials, but the molecular mechanisms driving its formation remain largely unknown. Here we show that the initial stage of process extension and axon ensheathment by oligodendrocytes requires dynamic actin filament assembly by the Arp2/3 complex. Unexpectedly, subsequent myelin wrapping coincides with the upregulation of actin disassembly proteins and rapid disassembly of the oligodendrocyte actin cytoskeleton and does not require Arp2/3. Inducing loss of actin filaments drives oligodendrocyte membrane spreading and myelin wrapping in vivo, and the actin disassembly factor gelsolin is required for normal wrapping. We show that myelin basic protein, a protein essential for CNS myelin wrapping whose role has been unclear, is required for actin disassembly, and its loss phenocopies loss of actin disassembly proteins. Together, these findings provide insight into the molecular mechanism of myelin wrapping and identify it as an actin-independent form of mammalian cell motility.

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Published In

Dev Cell

DOI

EISSN

1878-1551

Publication Date

July 27, 2015

Volume

34

Issue

2

Start / End Page

152 / 167

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • RNA, Small Interfering
  • RNA Interference
  • Optic Nerve
  • Oligodendroglia
  • Myelin Sheath
  • Myelin Basic Protein
  • Mice, Knockout
  • Mice, Inbred C57BL
 

Citation

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Zuchero, J. B., Fu, M.-M., Sloan, S. A., Ibrahim, A., Olson, A., Zaremba, A., … Barres, B. A. (2015). CNS myelin wrapping is driven by actin disassembly. Dev Cell, 34(2), 152–167. https://doi.org/10.1016/j.devcel.2015.06.011
Zuchero, J Bradley, Meng-Meng Fu, Steven A. Sloan, Adiljan Ibrahim, Andrew Olson, Anita Zaremba, Jason C. Dugas, et al. “CNS myelin wrapping is driven by actin disassembly.Dev Cell 34, no. 2 (July 27, 2015): 152–67. https://doi.org/10.1016/j.devcel.2015.06.011.
Zuchero JB, Fu M-M, Sloan SA, Ibrahim A, Olson A, Zaremba A, et al. CNS myelin wrapping is driven by actin disassembly. Dev Cell. 2015 Jul 27;34(2):152–67.
Zuchero, J. Bradley, et al. “CNS myelin wrapping is driven by actin disassembly.Dev Cell, vol. 34, no. 2, July 2015, pp. 152–67. Pubmed, doi:10.1016/j.devcel.2015.06.011.
Zuchero JB, Fu M-M, Sloan SA, Ibrahim A, Olson A, Zaremba A, Dugas JC, Wienbar S, Caprariello AV, Kantor C, Leonoudakis D, Lariosa-Willingham K, Kronenberg G, Gertz K, Soderling SH, Miller RH, Barres BA. CNS myelin wrapping is driven by actin disassembly. Dev Cell. 2015 Jul 27;34(2):152–167.
Journal cover image

Published In

Dev Cell

DOI

EISSN

1878-1551

Publication Date

July 27, 2015

Volume

34

Issue

2

Start / End Page

152 / 167

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • RNA, Small Interfering
  • RNA Interference
  • Optic Nerve
  • Oligodendroglia
  • Myelin Sheath
  • Myelin Basic Protein
  • Mice, Knockout
  • Mice, Inbred C57BL