CNS myelin wrapping is driven by actin disassembly.

Published

Journal Article

Myelin is essential in vertebrates for the rapid propagation of action potentials, but the molecular mechanisms driving its formation remain largely unknown. Here we show that the initial stage of process extension and axon ensheathment by oligodendrocytes requires dynamic actin filament assembly by the Arp2/3 complex. Unexpectedly, subsequent myelin wrapping coincides with the upregulation of actin disassembly proteins and rapid disassembly of the oligodendrocyte actin cytoskeleton and does not require Arp2/3. Inducing loss of actin filaments drives oligodendrocyte membrane spreading and myelin wrapping in vivo, and the actin disassembly factor gelsolin is required for normal wrapping. We show that myelin basic protein, a protein essential for CNS myelin wrapping whose role has been unclear, is required for actin disassembly, and its loss phenocopies loss of actin disassembly proteins. Together, these findings provide insight into the molecular mechanism of myelin wrapping and identify it as an actin-independent form of mammalian cell motility.

Full Text

Duke Authors

Cited Authors

  • Zuchero, JB; Fu, M-M; Sloan, SA; Ibrahim, A; Olson, A; Zaremba, A; Dugas, JC; Wienbar, S; Caprariello, AV; Kantor, C; Leonoudakis, D; Lariosa-Willingham, K; Kronenberg, G; Gertz, K; Soderling, SH; Miller, RH; Barres, BA

Published Date

  • July 27, 2015

Published In

Volume / Issue

  • 34 / 2

Start / End Page

  • 152 - 167

PubMed ID

  • 26166300

Pubmed Central ID

  • 26166300

Electronic International Standard Serial Number (EISSN)

  • 1878-1551

Digital Object Identifier (DOI)

  • 10.1016/j.devcel.2015.06.011

Language

  • eng

Conference Location

  • United States