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Metabolomic analysis reveals altered skeletal muscle amino acid and fatty acid handling in obese humans

Publication ,  Journal Article
Baker, PR; Boyle, KE; Koves, TR; Ilkayeva, OR; Muoio, DM; Houmard, JA; Friedman, JE
Published in: Obesity
May 1, 2015

Objective Investigate the effects of obesity and high-fat diet (HFD) exposure on fatty acid oxidation and TCA cycle intermediates and amino acids in skeletal muscle to better characterize energy metabolism. Methods Plasma and skeletal muscle metabolomic profiles were measured from lean and obese males before and after a 5-day HFD in the 4 h postprandial condition. Results At both time points, plasma short-chain acylcarnitine species (SCAC) were higher in the obese subjects, while the amino acids glycine, histidine, methionine, and citrulline were lower in skeletal muscle of obese subjects. Skeletal muscle medium-chain acylcarnitines (MCAC) C6, C8, C10:2, C10:1, C10, and C12:1 increased in obese subjects, but decreased in lean subjects, from pre- to post-HFD. Plasma content of C10:1 was also decreased in the lean but increased in the obese subjects from pre- to post-HFD. CD36 increased from pre- to post-HFD in obese but not lean subjects. Conclusions Lower skeletal muscle amino acid content and accumulation of plasma SCAC in obese subjects could reflect increased anaplerosis for TCA cycle intermediates, while accumulation of MCAC suggests limitations in β-oxidation. These measures may be important markers of or contributors to dysregulated metabolism observed in skeletal muscle of obese humans.

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Published In

Obesity

DOI

EISSN

1930-739X

ISSN

1930-7381

Publication Date

May 1, 2015

Volume

23

Issue

5

Start / End Page

981 / 988

Related Subject Headings

  • Endocrinology & Metabolism
 

Citation

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Chicago
ICMJE
MLA
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Baker, P. R., Boyle, K. E., Koves, T. R., Ilkayeva, O. R., Muoio, D. M., Houmard, J. A., & Friedman, J. E. (2015). Metabolomic analysis reveals altered skeletal muscle amino acid and fatty acid handling in obese humans. Obesity, 23(5), 981–988. https://doi.org/10.1002/oby.21046
Baker, P. R., K. E. Boyle, T. R. Koves, O. R. Ilkayeva, D. M. Muoio, J. A. Houmard, and J. E. Friedman. “Metabolomic analysis reveals altered skeletal muscle amino acid and fatty acid handling in obese humans.” Obesity 23, no. 5 (May 1, 2015): 981–88. https://doi.org/10.1002/oby.21046.
Baker PR, Boyle KE, Koves TR, Ilkayeva OR, Muoio DM, Houmard JA, et al. Metabolomic analysis reveals altered skeletal muscle amino acid and fatty acid handling in obese humans. Obesity. 2015 May 1;23(5):981–8.
Baker, P. R., et al. “Metabolomic analysis reveals altered skeletal muscle amino acid and fatty acid handling in obese humans.” Obesity, vol. 23, no. 5, May 2015, pp. 981–88. Scopus, doi:10.1002/oby.21046.
Baker PR, Boyle KE, Koves TR, Ilkayeva OR, Muoio DM, Houmard JA, Friedman JE. Metabolomic analysis reveals altered skeletal muscle amino acid and fatty acid handling in obese humans. Obesity. 2015 May 1;23(5):981–988.
Journal cover image

Published In

Obesity

DOI

EISSN

1930-739X

ISSN

1930-7381

Publication Date

May 1, 2015

Volume

23

Issue

5

Start / End Page

981 / 988

Related Subject Headings

  • Endocrinology & Metabolism