A microRNA miR-34a-regulated bimodal switch targets Notch in colon cancer stem cells.

Journal Article

microRNAs regulate developmental cell-fate decisions, tissue homeostasis, and oncogenesis in distinct ways relative to proteins. Here, we show that the tumor suppressor microRNA miR-34a is a cell-fate determinant in early-stage dividing colon cancer stem cells (CCSCs). In pair-cell assays, miR-34a distributes at high levels in differentiating progeny, whereas low levels of miR-34a demarcate self-renewing CCSCs. Moreover, miR-34a loss of function and gain of function alter the balance between self-renewal versus differentiation both in vitro and in vivo. Mechanistically, miR-34a sequesters Notch1 mRNA to generate a sharp threshold response where a bimodal Notch signal specifies the choice between self-renewal and differentiation. In contrast, the canonical cell-fate determinant Numb regulates Notch levels in a continuously graded manner. Altogether, our findings highlight a unique microRNA-regulated mechanism that converts noisy input into a toggle switch for robust cell-fate decisions in CCSCs.

Full Text

Duke Authors

Cited Authors

  • Bu, P; Chen, K-Y; Chen, JH; Wang, L; Walters, J; Shin, YJ; Goerger, JP; Sun, J; Witherspoon, M; Rakhilin, N; Li, J; Yang, H; Milsom, J; Lee, S; Zipfel, W; Jin, MM; Gümüş, ZH; Lipkin, SM; Shen, X

Published Date

  • May 2013

Published In

Volume / Issue

  • 12 / 5

Start / End Page

  • 602 - 615

PubMed ID

  • 23642368

Electronic International Standard Serial Number (EISSN)

  • 1875-9777

International Standard Serial Number (ISSN)

  • 1934-5909

Digital Object Identifier (DOI)

  • 10.1016/j.stem.2013.03.002

Language

  • eng